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                June 
                - 2010 
 
                
                
                Original Research 
                
                1.   
                
                
                Bovine cysticercosis in cattle slaughtered at Jimma municipal 
                abattoir, south western Ethiopia: prevalence, cyst viability and 
                its socio-economic importance - 
                
                Bekele Megersa, Eliyas Tesfaye, Alemayehu Regassa, Rahmeto Abebe, 
                Fufa AbunnaVet World. 2010; 3(6): 257-262
 
                
                A cross sectional study 
                was conducted during November 2008 to March 2009 to estimate the 
                prevalence of Cysticercosis in animals, Taeniasis in human and 
                estimate the worth of taeniasis treatment in Jimma town. Active 
                abattoir survey, questionnaire survey and inventory of 
                pharmaceutical shops were performed to accomplish the study. Of 
                the total of 500 inspected animals, 22 animals had varying 
                number of C. bovis giving an over all prevalence 4.4% (22/500). 
                Anatomical distribution of the cyst showed that highest 
                proportions of C. bovis cyst were observed in shoulder muscle, 
                followed by tongue, heart and masseter muscle. Of the total of 
                114 C.bovis collected during the inspection, 49(42.9%) were 
                found to be a live while others (57.0%) were degenerative cyst. 
                Of the total 60 interviewed respondents, 56.7% (34/60) had 
                contracted T.saginata infection, of which, 95% and (5%) cases 
                reported using modern drugs and traditional drugs, respectively. 
                The majority of the respondent had an experience of row meat 
                consumption as a result of traditional and cultural practice. 
                Human Taeniasis prevalence showed significant difference 
                (p<0.05) with age groups, meat consumption, sex and use of 
                spice. Accordingly adult individual (OR=47.4), frequent raw meat 
                consumers (OR=18.4), spice users (OR=7.0) and male (OR=5.0) had 
                higher odds acquiring Taeniasis than children, occasional meat 
                consumer, non spice users and females, respectively. In this 
                analysis there was no significance difference between religion, 
                education status, occupational risks and marital status 
                (p>0.05). An inventory of pharmaceutical shops (pharmacies, drug 
                stores and rural drug vendors) revealed a total of 103,596 adult 
                taeniacidal drug doses worthing a total of 222,706 Eth. Birr 
                (22,270.6 USD) during two years of 2007 and 2008. Vermox and 
                Niclosamide were the most frequently sold drug for the treatment 
                of taeniasis, while Praziquantel was least sold drug. The 
                findings of this study including prevalence of C. bovis, 
                questionnaire survey of taeniasis prevalence and the 
                pharmaceutical shops inventory indicated the importance of 
                Cysticercosis and Taneniasis both in public health and 
                economical aspects. Therefore, due attention should be given to 
                the public awareness and strict routine meat inspection in order 
                to safe guard the public health.
 Keywords: 
                Abattoir, C. bovis/Taeniasis, Cattle, Prevalence, Ethiopia
 
                » Abstract 
                & References » 
                PDF Fulltext» doi: 10.5455/vetworld.2010.257-262 
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                Original Research 
                
                2.   
                
                
                Ovarian follicular fluid constituents in relation to stage of 
                estrus cycle and size of the follicle in buffalo - 
                M. R.
                Abd Ellah, H. A. Hussein and D. R. DerarVet World. 2010; 3(6): 263-267
 
                
                The goal of the present study was to evaluate the difference in 
                constituent of the ovarian follicular fluid in different stages 
                of the estrus cycle and in medium and large sized follicle and 
                also to evaluate the relation between serum and follicular fluid 
                constituents in cyclic buffalos. A total of 34 clinically 
                healthy buffalo (Bubals bubals), aged 7-10 years, were sent for 
                slaughter in Moesha Abattoir, Assiut province in winter 2009. 
                Blood samples and the whole genital tract of each animal were 
                collected. The stage of the cycle (proestrus n= 8, estrus n= 7, 
                metestrus n= 7 and diestrus n= 12) was determined post mortem. 
                Biochemical analysis of serum and follicular fluid was performed 
                through measuring total protein, albumin, chloride, potassium, 
                phosphorus, magnesium, glucose, cholesterol, triglyceride, urea, 
                creatinine levels and lactate dehydrogenase (LDH) activity. 
                Results of the present study revealed that during the estrus 
                cycle, only follicular triglyceride, urea, creatinine and 
                phosphorus level showed significant changes. A positive 
                correlation was found between follicular albumin, phosphorus 
                levels and follicular diameter. Total protein, albumin, 
                globulins, glucose, chloride and creatinine were significantly 
                higher in the serum than that in the follicular fluid. 
                Follicular triglyceride level and potassium level were 
                significantly higher than serum level. Follicular LDH activity 
                was higher in large sized follicle than small sized one. Further 
                studies are required to elucidate the relation between 
                concentration of urea and creatinine in the follicular fluid and 
                oocyte viability.
 Key words: estrus, buffalo, follicular fluid, serum.
 
                » Abstract » 
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                Original Research 
                            
                            3. 
                            
                               Detection of total and 
                            pathogenic Vibrio parahaemolyticus by Polymerase 
                            chain reaction using toxR, tdh and trh genes 
                            - Subhashini Nelapati and N. Krishnaiah 
                             
                            
							Vet
                            World 3(6):268-271   
                            
                            The levels of total and 
                            pathogenic Vibrio parahaemolyticus were estimated in 
                            105 samples (each 35 of fresh water fish, sea fish 
                            and mutton) using cultural and polymerase chain 
                            reaction (PCR) methods by amplification of toxR gene 
                            for total V. parahaemolyticus, tdh and trh genes for 
                            pathogenic V. parahaemolyticus. Out of 105 samples, 
                            30 (28.6%), 39 (37.1%) samples gave positive results 
                            for total V. parahaemolyticus by cultural and PCR 
                            methods respectively. Out of 39 samples positive for 
                            PCR 6 (5.7%), 28 (26.6%) and 3 (8.6%) were positive 
                            for tdh, trh and both tdh and trh respectively.
 Keywords: Vibrio parahaemolyticus, PCR,. 
                            Gene, Laboratory Technique, Pathogenic Organism.
 
                              
                            
                            
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                Original Research 
                
                4.   
                
                
                Efficacy of various antiprotozoal drugs on bovine babesiosis, 
                anaplasmosis and thileriosis -
                
                
                Nasreen Akhter, Chamon Lal, Javaid Ali Gadahi, Khuda Bux 
                Mirbahar, and Mohammad Ismail Memon Vet World. 2010; 3(6): 272-274
 
                
                The present study was conducted to check out the efficacy of 
                various antiprotozoal drugs on bovine babesiosis, anaplasmosis 
                and theleriosis. 38 buffaloes naturaly infected with anaplasma, 
                babesia and theileria were selected and divided into four 
                groups. Group A (10), B (10), C (08) and D (10) infected with 
                anaplasmosis, babesiosis, theileriosis, and Infected Control (anaplasmosis 
                (4), anaplasmosis + babesiosis (1 each), babesiosis (2) and 
                theileriosis (2) respectively. Group A was further subdivided 
                into A-I (5 animals with pure Anaplasmosis) and A-II (5 animals 
                with mixed infection of Anaplasmosis and Babesiosis). Group-A 
                was treated with a single dose of imidocarb dipropionate (3 mg / 
                kg body weight). In Group A-I one animal recovered completely, 
                02 moderately while remaining 02 animals did not respond. The 
                later 02 animals recovered completely after additional 02 doses 
                of 4 mg/kg body weight. In case of group A-II complete recovery 
                was recorded in one animal, moderate recovery in three animals 
                while one animal showed no responsel. Group B was treated with 
                Diminazene acceturate (3. 5 mg / kg body weight) administered as 
                a single dose. 6 animals recovered completely, 02 moderately 
                while 02 animals showed no response. Group C was treated with a 
                single dose of Buparvaqune (2.5 mg / kg body weight), complete 
                recovery was recorded in 4 animals, 2 animals showed no 
                response, while 2 animals with severe infection died after 24 
                hrs. .
 Keywords: Babesiosis, anaplasmosis, theileriosis, drug 
                efficacy, buffalo
 
                » Abstract » 
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                Original Research 
                
                5.   
                
                
                Induced aflatoxin and carbon tetrachloride toxicity on gravid 
                uterus of rabbits – A patho-morphological study -
                
                
                Panchakshari Sankh, K. J. Ananda and B. H. Manjunatha PrabhuVet World. 2010; 3(6): 275-276
 
                
                Uterus of the pregnant 
                rabbits, treated with aflatoxin showed resorption of foetuses. 
                Histologically, extensive haemorrhages in the endometrial and 
                parametrial areas and presence of extensive fibrosis of mucosa 
                and musclaris were observed. In combined treatment with 
                aflatoxin and carbon tetrachloride resorption of foetuses was 
                noticed. Hyperplastic or metaplastic changes of lining 
                epithelium of the endometrial mucosa was observed. The lining 
                epithelium usually was that of cuboidal type. Multiple 
                haemorrhages in the submucosa were observed. In pure carbon 
                tetra chloride treatment, there was no resorption of foetuses, 
                but extensive fibroblastic proliferation in uterine muscularis 
                and as wall as periglandular and interstitial proliferation of 
                fibroblasts in the endometrium were seen.
 Key words: 
                Pregnant rabbits, Aflatoxin, Carbon tetrachloride, Uterus
 
                » Abstract » 
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                Original Research 
                
                6.   
                
                
                Effect of temperature and storage time on hepatobiliary enzyme 
                activities in goat serum -
                
                
                P. D. Divya and K.K. JayavardhananVet World. 2010; 3(6): 277-279
 
                  
                
                The present study was 
                designed and conducted to choose an ideal storage condition for 
                goat sera samples meant for the assay of hepatobiliary enzymes 
                such as, alanine aminotransferases(ALT), aspartate 
                aminotransferases (AST), alkaline phosphatase (ALP) and gamma 
                glutamyltran-sferases (GGT) by storing at room temperature, 4 ºC 
                and -20 ºC up to 14 days. Gamma glutamyltransferase was found to 
                be the most stable enzyme in all the three storage conditions 
                through out the study period. Alanine aminotransferase was 
                stable only up to 8 days at 4 ºC whereas marked stability was 
                noticed at -20 ºC and room temperature as long as 14 days. 
                Aspartate aminotransferase was more stable at -20 ºC up to14 
                days and 11 days at 4 ºC whereas at room temperature only 2 
                days. Alkaline phosphatase showed great variation upon storage 
                as compared to other hepatobiliary enzymes and it is suggested 
                that its estimation should be performed in fresh serum samples 
                to get a more accurate result. From these results it is 
                therefore advisable to consider stability of each serum 
                hepatobiliary enzymes for different animals separately before 
                preserving sera samples to get more valid and reliable result.Key words: storage 
                stability, crossbred goats, hepatobiliary enzymes
 
                » Abstract » 
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                Original Research 
                
                7.   
                
                
                Effect of dietary supplementation of shatavari (Asparagus 
                racemosus wild) on heamatobiochemical parameters of broilers - 
                 
                
                D. H.
                Rekhate, Smita Ukey, Leena N. Mangle and B. S. DeshmukhVet World. 2010; 3(6): 280-281
 
                  
                
                Eighty, day old straight 
                run commercial broilers chicks were randomly distributed into 
                four groups (T1, T2, T3 and T4) of two replications having ten 
                chicks in each, reared on deep litter system upto 8 weeks of 
                age. The chicks in all the treatment groups were fed basal diet 
                T1 control and T2, T3 and T4 groups were fed basal diet + 0.5, 
                1.00 and 1.5 per cent Shatavari root powder (SRP) respectively. 
                Significant (P<0.01) improvement in live body weight of broilers 
                was observed between the treatments. Better FCE was observed 
                with the increase in SRP. The digestibility of protein found 
                significantly (P< 0.01) better with higher inclusion level of 
                SRP. Hb, total serum protein, albumin and globulin revealed 
                significant (P< 0.01) variation between the treatments. It was 
                concluded that supplementation of Shatavari root powder at 0.5, 
                1.00 and 1.5 per cent level improves growth performance and 
                general health status of broilers however, 1.0 per cent level 
                could be more economical.Key words: 
                broilers, Shatavari, haematobiochemical.
 
                » Abstract » 
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                Original Research 
                
                8.   
                
                
                
                Epidemiology of canine mammary gland tumours in Gujarat - 
                M.A. Dhami, P.H. Tank, A.S. Karle, H.S.Vedpathak and A.S.BhatiaVet World. 2010; 3(6): 282-285
 
                
                Last three years’ 
                (2006-2009) retrospective information was retrieved by 
                scrutinizing the data bank of the College Hospital. During this 
                period 8337 canine cases were registered. Amongst them 2070 
                cases were referred to Department of Surgery for further 
                surgical treatment. Out of 2070 canine cases, 158 cases (7.63 %) 
                had various neoplastic condition and amongst them 63 cases 
                (39.87 %) were of mammary gland tumours. The overall incidence 
                of canine mammary gland tumours was 0.75 per cent of the total 
                canine cases. The highest occurrence of canine mammary tumour 
                cases was found in the dogs aged 8 to 12 years (49.21 %) 
                followed by those aged 4 to 8 years (33.33 %). It was least 
                (4.76 %) in youger dogs less than 4 years and intermediate 
                (12.70 %) in older dogs above 12 years of age. Breed-wise higher 
                occurrence was found in German Shepherd and Pomeranian as 
                compared to other breeds.
 Key words: Canine, 
                Mammary tumour, Epidemiology, Age and Breed effect.
 
                » Abstract » 
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                Original Research 
                
                9.   
                
                
                Influence of ovarian side and initiation time of first 
                aspiration with relation of transvaginal follicular aspiration 
                in HF x Sahiwal cows - 
                V. S.
                Suthar, R. G. Shah, S. P. Singh, R. Kasiraj and A. J. DhamiVet World. 2010; 3(6): 286-288
 
                
                This study was carried 
                out to observe the influence of ovarian side and initiation time 
                of first aspiration during OPU programme in HF X Sahiwal cows. 
                Eight cows were randomly divided in to two groups (A and B) each 
                consisting of 4 donors. Follicular aspiration was initiated on 
                day 4 (first follicular wave) and day 13 (second follicular 
                wave) of estrous cycle, respectively. From group-A donors, total 
                200 follicles were aspirated in eight sessions with a mean of 
                6.25 ± 2.05 per animal per session of which 52.50 (105/200) and 
                47.50 (95/200) per cent follicles were from left and right 
                ovary, respectively. The mean numbers of small, medium and large 
                follicles aspirated were 2.06 ± 0.87, 2.65 ± 0.88 and 1.53 ± 
                0.91 with the recovery rate of 33.0 (66/200) (3–5 mm), 42.50 
                (85/200) (6–9 mm) and 24.50 (49/200) (=10 mm) per cent, 
                respectively. From group-B donors, total 179 follicles were 
                aspirated of which 48.60 (87/179) and 51.40 (92/179) per cent 
                follicles were from left and right ovary, respectively. The mean 
                number of aspirated follicles per cow per session from group-B 
                donor cows was 5.59 ± 1.03 and the mean numbers of small, medium 
                and large follicles aspirated 1.87 ± 0.96, 2.5 ± 0.66 and 1.25 ± 
                0.64 with the recovery rate of 34.07 (61/179), 44.94 (80/179) 
                and 21.22 (38/179) per cent, respectively. The number of 
                follicles from left and right ovaries of different categories 
                among group A and B donor cows did not differ significantly 
                (P>0.05). This study showed that there cows no any influence of 
                ovarian side and initiation time on follicular aspiration.
 Keywords: Ovary, 
                Transvaginal Follicular Aspiration, Follicle, Reproductive 
                organ.
 
                » Abstract » 
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                Original Research 
                
                10.   
                
                
                Effect of sunflower cake inclusion on certain blood biochemicals 
                of Japanese quails - 
                D. H.
                Rekhate, V.M. Patil, Leena N. Mangle and B. S. DeshmukhVet World. 2010; 3(6): 289-290
 
                
                A week old 120, Japanese 
                quail chicks were equally and randomly distributed into four 
                different treatment groups (T0, T1, T2 and T3), of three 
                replicates having 10 chicks in each. All the chicks were reared 
                on saw dust litter upto six weeks of age. The chicks in control 
                group-T0 were fed corn-soya based diet with multi- enzyme and 
                group T1, T2 and T3 were fed on diets containing 10, 20 and 30 
                per cent replaced soybean cake by sunflower cake with 
                multi-enzyme supplementation, respectively. The significant (P< 
                0.01) differences were noticed between the treatments for weekly 
                live body weights upto sixth week. The blood-biochemicals viz., 
                serum glucose, serum total protein and serum cholesterol level 
                did not varied significantly. It was concluded that inclusion of 
                sunflower cake do not have any adverse effect on 
                blood-biochemical parameters however supplementation of 
                multi-enzyme improves numerically the growth performance of 
                Japanese quails.
 Key Words: Japanese 
                quails, Sunflower cake, Multi-enzyme, Blood-Biochemical profile.
 
                » Abstract » 
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                Original Research 
                
                11. 
                
                
                Effect of different management system on haemato-biochemical 
                profile in quail -
                
                
                Vijay Kumar, R.K. Verma, Satish Kumar, S.K. Singh and Ramesh 
                Kumar SinghVet World. 2010; 3(6): 291-292
 
                
                A very little information 
                is available in literature on management of Japanese quail (Couturnix 
                Couturnix Japonica) in different housing system (cage system and 
                deep litter system) of management. The average weekly body 
                weight gain was significantly higher in deep litter system 
                (34±0.43gm) than cage (12.71±0.41gm) system at the 3rd week of 
                age. The average daily feed consumption by individual quails was 
                higher in cage (12.71±2.10) than deep litter system (11.84±1.47) 
                during 0-6 weeks of age. The haematobiochemical profile viz Hb (gm%),TEC 
                (106/µl ),PCV(%),TLC(103/ µl)along with biochemical studies as 
                blood sugar (mg/dl), total serum protein (gm/dl),serum calcium 
                (mg/100ml) and serum phosphorus (mg/dl) were well within the 
                normal health of quail under both cage and deep litter system of 
                management.
 Keywords: 
                Management System, Quail, Hemato-biochemical profile, Cage sytem, 
                Deep Litter system.
 
                » Abstract » 
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                Original Research 
                
                12. 
                
                
                Treatment of post partum anestrous in Osmanabadi goats with 
                Janova -
                
                
                A. D. Patil, D.S. Raghuwanshi, K. Ravikanth and Shivi Maini Vet World. 2010; 3(6): 293-294
 
                  
                
                The present study was 
                conducted in a flock of Osmanabadi Goats reported with the 
                history of post-partum anestrous. The experimental goats were 
                selected from Goat farm unit, College of Veterinary & Animal 
                Sciences Udgir, Maharashtra, India. A total of 20 Osmanabadi 
                Goats were divided into two equal groups (I & II), (n=10). 
                Group-I served as untreated control group. Group II were 
                administered orally with caplet Janova@2 cap/day/goat for three 
                days. The efficacy of treatment in group II was 80 percent (%) 
                induction of oestrus within 96.37 ? 1.40 hrs and 62.50 % 
                conceived with 1.8 services per conception whereas in control 
                group I 30% oestrus was observed within 185.5 ? 10.89 hrs and 
                33.33 % conceived with 3.00 services per conception. It may be 
                concluded that administration of polyherbal formulation Janova 
                has lead to induction of fertile post partum oestrus and higher 
                conception rate in Osmanabadi goats. Key words: herbal, 
                heat inducer, anestrous, Osmanabadi goat
 
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                Case Report 
                
                13. 
                
                
                Ethno-veterinary drug therapy for ear mange in sheep -
                
                
                S. D. Hagawane, G.R.Rajurkar and S.B.Shinde Vet World. 2010; 3(6): 295-296
 
                
                Sheep from two flocks 
                showed scab lesions around nostrils, eyes and on the face with 
                facial alopecia and thickening of the skin. The case was 
                confirmed as ear mite (Psorcopt ovis) by microscopic examination 
                of skin scrapings. Percent incidence in flock I and Flock II 
                recorded was 12.5, 11.4 respectively. Affected sheep were 
                divided into two groups each containing nine animals. Group I 
                was treated with preparation containing 50ml Azadirachta indica 
                oil, 50ml Pogamia pinnata oil, 25gm Camphor, 50gm Sulphur powder 
                and 500ml coconut oil applied on an affected skin twice a day 
                for 15 days and Group II treated with preparation having 50gm of 
                Curcuma longa rhizome and 25gm of Azadirachta indica oil applied 
                once a day for 15 days. The first preparation was found highly 
                effective against ear mange in sheep. It was found to be very 
                convenient for use in field conditions, did not cause any 
                adverse reaction locally as well as systemically.
 Keywords: 
                Ethnoveterinary, Ear mange, Sheep, Drug, Therapy.
 
                » Abstract » 
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                Review Article 
                
                14. 
                
                
                
                Gallinacin and fowlicidin: two promising antimicrobial peptides 
                in chickens—A review - 
                C. S. 
                Mukhopadhyay, Ravi Kumar and G. S. Brah Vet World. 2010; 3(6): 297-300
 
                
                Antimicrobial peptides 
                (AMP) which have been identified in almost all groups of 
                organisms, are the small cationic molecules that recognize the 
                pathogen associated molecular patterns of the microbes. In 
                chicken two main AMPs that play significant roles in bolstering 
                the innate immunity are gallinacins and fowlicidins, which are 
                the functional analogues of the mammalian beta-defensins and 
                cathelicidins. Gallinacin identifies the Gram negative bacteria 
                while fowlicidin exerts broad spectral activity. The basic 
                mechanism of action is by far similar in both groups of AMPs. 
                The ‘docking sites’ of these antimicrobial peptides includes the 
                “lipid A” moiety of lipo polysaccharides, lipo-teichoic acids, 
                anionic membrane phospholipids on bacterial surfaces. These AMPs 
                block the DNA replication and protein synthesis in bacteria 
                causing death of the microbe. Researchers have identified 
                reproducible molecular markers of those peptides for selection 
                of disease resistant stock of chickens.
 Key words: 
                Gallinacin, Fowlicidin, Antimicrobial peptides, Chicken, Innate 
                immunity.
 
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