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Research
2.
Seroprevalence of avian leukosis virus antigen
using ELISA technique in exotic broilers and
nigerian local chickens in Zaria, Nigeria -
N. A. Sani, S. B. Oladele, M. A. Raji, D. G.
Ibrahim
Vet World. 2011; 4(8): 345-348
doi: 10.5455/vetworld.2011.345-348
Abstract
In an attempt to determine the seroprevalence of
avian leukosis virus (ALV) in exotic broiler
chickens and Nigerian local chickens in Zaria,
Nigeria, a total of 600 sera (300 from exotic
broiler chickens and 300 from Nigerian local
chickens), obtained from the live bird market in
Zaria, Nigeria, were tested for ALV p27 antigen by
the antigen capture - enzyme linked immunosorbent
assay (ac-ELISA) technique. The age range of the
Nigerian local chickens sampled in this study was
6 – 24 months, while that of the exotic broiler
chickens used in this study was 2-3 months.
Fourteen out of the 300 sera obtained from the
exotic broiler chickens tested positive to ALV p27
antigen, which represents 4.70%, while 180 of the
300 Nigerian local chicken sera were confirmed
positive to the antigen, representing 60.00%.
Thirteen (92.86%) of the fourteen sera from the
exotic broiler chickens were lowly positive (ELISA
Units range of 10-20%) to ALV p27 antigen, while
only one (7.14%) serum sample was moderately
positive to ALV p27 antigen with an ELISA Unit of
29.33%. Of the 180 sera from the Nigerian local
chickens that tested positive to ALV p27 antigen ,
79 (43.89%) were lowly positive with ELISA Units
ranging from 10.67% to 21.33%, while 101 (56.11%)
serum samples were moderately positive to ALV p27
antigen with ELISA Units ranging from 28.0% to
73.33%. A higher seroprevalence of ALV was
detected in Nigerian local chickens than the
exotic broiler chickens.
Keywords:
Seroprevalence, Avian leukosis virus, ELISA, Zaria.
