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Research
1.
Survival and infectivity studies of in-vitro
cultivated larvae of Haemonchus contortus
in sheep and goats in Nigeria -
A O Sonibare, H A Kumshe, E A Okewole, A 0 Talabi,
O. O. Alaka, E B Otesile, R A Joshua
Vet World. 2011; 4(12): 533-536
doi: 10.5455/vetworld.2011.533-536
Abstract
The survival and infectivity of indigenous
isolates of Haemonchus contortus and in vitro
cultivated infective larvae (L3) were studied.
Active motile gravid females, adult and immature
parasites were morphologically and
morphometrically characterized and isolated from
Haemonchus infected goats. These were subsequently
inoculated into the abomasum of parasite free
lambs through left sided laparatomy for production
of fertile eggs to be used for coproculture.
Harvested faeces from these lambs were cultured in
a nutritive medium and kept in the incubator at
320C and room temperature 24-280C to obtain in
vitro cultivated L3 of H. contortus. The
infectivity and survival of in vitro cultivated L3
stored in refrigerator at 4-80C and under room
temperature 24-280C were evaluated. Incubation
period of coproculture was observed to be shorter
in medium at 320C than under room temperature
24-280C. The storage of in vitro cultivated L3
under different temperature range showed that the
quantity of active larvae in a given volume
decreased at p<0.05 from the 5th day under 24-280C
while at 4-80C became significantly lower from
20th day. Infectivity of isolate kept at 4-80C was
sustained for 18-20 days while at 24-280C it was
maintained for 10-12 days. Laboratory in-vitro
cultivated L3 of H. contortus had limited
infectivity and larvae mortality increase with
time under different temperatures. Keywords:
Indigenous goats, Haemonchus contortus, in
vitro cultivated L3, storage, survival and
infectivity
