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Research
8.
Inv A gene specific PCR for detection of
Salmonella from broilers - Malmarugan Shanmugasamy, Thenmozhi Velayutham,
Johnson Rajeswar
Vet World. 2011; 4(12): 562-564
doi: 10.5455/vetworld.2011.562-564
Abstract
Poultry meat has been identified as one of the
principal foodborne source of Salmonella. In this
preliminary study the prevalence of Salmonella spp.
contamination of broiler carcasses, were
determined. Sixty samples were collected from
poultry carcasses from the commercial broiler
slaughtering facility in Namakkal, Tamil Nadu. The
presence of Salmonella spp in collected samples
was assessed by performing the pre-enrichment and
enrichment culture, followed by PCR assay. The
primers were selected from the invA gene specific
for the detection of Salmonella spp. In this study
8.3% of poultry carcasses were found to be
contaminated with Salmonella spp. In order to
provide a more accurate profile of the prevalence
of Salmonella spp in broiler carcasses, it is
pertinent to use inv A gene specific PCR method
that could be considered as an appropriate
alternative to conventional culture method.
Keywords: Salmonella, broiler, polymerase
chain reaction (PCR), invA Gene
