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              Research
                            
              
                            
                            5.  Recovery status of 
                            bacteriophages of different livestock farms of 
                            veterinary college, Adhartal, Jabalpur, India - Sanjay Shukla, S. D. Hirpurkar
                            Vet World. 2011; 4(3): 117-119
              
              
               
              
              
              doi: 
              10.5455/vetworld.2011.117-119
              
              
               
              
              
              
   
 
              
               
               
               
              
              
              Abstract
              
                            
                            
                            Study was conducted to know the presence of 
                            bacteriophage in sewage material which can play a 
                            very important role during therapy against the some 
                            antibiotic resistance organisms. During study waste 
                            water samples were collected from different depths 
                            of the wastewater collection tanks located in 
                            livestock farms of different species (Cattle, pig, 
                            goat and poultry). These samples were subjected 
                            primarily to rapid detection by streak plate method 
                            for the detection of lytic activity followed by 
                            primary isolation of phage against two most common 
                            bacteria of environment, namely, B. subtilis and E. 
                            coli by Double agar layer (DAL) method. Recovery of 
                            phages was maximum from pig feces (67%) followed by 
                            dairy cattle farm waste (63%), buffalo farm waste 
                            (50%), goat farm waste (13%). 
                            
                            
                            Keywords: 
                            Double agar layer (DAL), B. subtilis and E. coli, 
                            Bacteriophage, Sewage, Waste Water, Livestock Farm