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Research
6.
Molecular
characterization of lingual antimicrobial peptide in
the female reproductive tract of buffalo - Dhanya Joseph, Tukaram More
Vet World. 2011; 4(3): 120-123
doi:
10.5455/vetworld.2011.120-123
Abstract
Bubalus bubalis (Ruminantia: Bovidae, Bovinae) is an
economically important animal of many Asian
countries, making significant contribution to milk
and meat production. Sub clinical infection of the
reproductive tract is one of the important causes
for reduced reproductive efficiency in dairy herd of
buffaloes. Antimicrobial peptides are component of
innate immune system which helps in augmenting the
resistance to infection at epithelial surfaces e.g
reproductive tract epithelium. In this study we have
identified a ß-defensin called Lingual Antimicrobial
Peptide (LAP) in buffalo reproductive tract.
Interestingly the gene was 100 % identical to the
LAP isolated from the tongue epithelium of Bos
taurus. The 195 bp cDNA of LAP codes for 64 amino
acids and of which 50% are cationic amino acids.
Phylogenetic studies indicate that LAP of
reproductive epithelium of buffalo is different from
other beta defensins isolated from the various
tissues of same species, but all beta defensin were
found to have the same progenitor gene. It is
concluded that buffalo reproductive tract epithelium
lining contains LAP.
Keywords: Antimicrobial
Peptide, Reproduction, Buffalo, Milk Production,
Meat Production, Amino acid.
