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Research
5.
Serodiagnosis of Oesophagostomum Columbianum
infection in goats using indirect ELISA - Bashir Ahmad Lone, M. Z. Chisti, Fayaz Ahmad,
Hidayatullah Tak
Vet World. 2011; 4(11): 503-506
doi: 10.5455/vetworld.2011.503-506
Abstract
To determine the seroprevalence of Oesophagostomum
columbianum antibodies in goats in Kashmnir, a
study was conducted in Srinagar and Ganderbal
Districts, located in central parts of Kashmir
valley. ELISA was standardized and evaluated to
detect goat Oesophagostomosis in experimental and
clinical cases using somatic whole adult antigen
of Oesophagostomum columbianum. Plate ELISA was
standardized using 5µg/well antigen concentration
with 1:100 and 1:1000 of sera and conjugate
dilution. Indirect plate ELISA was able to
demonstrate the antibody titer at different weeks
post infection in experimental goats. A comparison
of plate ELISA on suspected field sera and faecal
sample examination by floatation method revealed
that 68 (34.34%) samples were found to be positive
using ELISA and twelve by faecal examination. The
highest prevalence was found in lower age groups
in both the seasons, with the increase in age, the
infection level decreased. The prevalence was
higher in wet season as compared to dry season.
The results were stastically significant (P <
0.05). No sex predisposition was seen. Sensitivity
and specificity of ELISA was 80.0%, and 21.42%,
respectively. This test is therefore quite
sensitive for clinical cases; an early diagnosis,
however lacks specificity of faecal examination
were 10.81% and 90.0%, respectively.
Keywords:
ELISA, Oesophagostomum columbianum, Goat,
Somatic antigen
