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Research
2.
Studies of the antigenic relationships between
bluetongue virus serotypes 2, 9 & 15 isolated in
Andhra Pradesh, India - Deepthi Balam, Sreenivasulu Daggupati, Himaja
Maddireddy
Vet World. 2011; 4(10): 444-448
doi: 10.5455/vetworld.2011.444-448
Abstract
The presence of multiple serotypes of the
midge-borne bluetongue virus and lack of effective
vaccine are the major impediments in controlling
bluetongue in sheep. Attempts are being made to
develop a vaccine employing the available
serotypes to control the disease in the state.
Hence, it is essential to identify the antigenic
relationships among the serotypes to identify the
candidate strains to be incorporated in the
preparation of vaccine. To understand the
antigenic relationships between Bluetongue virus
-2, 9 and 15 serotypes, the viruses were
propagated in BHK21 cell lines, purified using PEG
precipitation method and purified virus used to
raise hyper immune serum in rabbits. Neutralizing
antibodies for the BTV serotypes were detected by
day 21 PI. Reciprocal cross neutralization test
was employed to determine the R% values between
BTV-2, 9 and 15 which indicated the extent of
antigenic relationships among the serotypes. R%
value between BTV-2 and BTV-9 was recorded as 2.8.
R% value of 3.53 and 2.8 were observed between
BTV-2 & 15 and BTV-9 & 15 respectively. The R%
values recorded in the present study revealed a
weak antigenic relationship between the BTV
serotypes, indicating that the serotypes are
highly divergent.
Keywords: Bluetongue
virus; serotypes; antigenic relationship.
