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Research
4.
Assessment of sprague dawley rats as biomodel in
two antigenotoxicity assays -
D. F Arencibia-Arrebola, L. A Rosario-Fernández,
Y. E Suárez-Fernández, A Vidal-Novoa
Vet World. 2011; 4(10): 455-460
doi: 10.5455/veworld.2011.455-460
Abstract
The discovery of new drugs with antigenotoxic
effect constitutes in our days a prioritized line
of research. In this work was evaluated the
response of Sprague Dawley rats (both sexes)
against cyclophosphamide and bleomycin by means of
alkaline comet assay of peripheral blood
leukocytes and micronuclei in bone marrow cells
assay as antigenotoxicy biomodel. Higher induction
of the strand breaks (SB) and alkali-labile sites
formation on DNA damage were obtained with the use
of the cyclophosphamide and bleomycin, both in the
design of 48 and 24 hr administration before the
euthanasia. In the mironucleis assay was obtained
high results of induction the micronucleus
formation in bone marrow cells with
cyclophosphamide was administered 48 and 24 hr
before euthanasia in both sexes. These results are
useful in studies of drugs evaluation that they
have not been explored in to the in vivo
antigenotoxicity environment.
Keywords:
Sprague Dawley, rats, biomodel, antigenotoxicity
assays, micronucleus assay, comet assay.
