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Original Research
4.
Prevention of tri-nitrobenzene of sulfonic
acid-induced colitis in chicken by using extract of
Aloe vera -
Motamed Elsayed Mahmoud, Khaled M. A. Hassanein
Vet World. 2012; 5(8): 469-476
doi:
10.5455/vetworld.2012.
469-476
Abstract
Aim:
Aloe vera, species of succulent plant in the genus
Aloe, has multiple clinical activities and used
routinely to accelerate wound healing. The present
study was designed to investigate the
anti-inflammatory effect of Aloe vera extracts (AVE)
in vitro and in vivo.
Materials and Methods:
The effect of crude AVE on inducible nitric oxide
production by LPS/IFNg-stimulated cultured
macrophages was evaluated. The therapeutic effect of
administering crude Aloe vera extracts (100 mg/kg
b.w.) on the development of tri-nitrobenzene of
sulfonic acid (TNBS)-induced colitis (40 mg/kg b.
w.) in chicken was also investigated. Chicken is a
valuable model for this purpose because it showed
preference to bitter taste of Aloe vera. Diverse
clinical pictures of the colitis including weight
loss, diarrhea and histopathological changes were
evaluated.
Results: Nitrite production by
LPS/IFNg-stimulated macrophages was maximally
reduced by adding of AVE (100 μg/ml). This result
suggests a direct inhibitory effect of AVE on the
inflammatory cells. Chicks treated orally with AVE
showed improvement of the histological signs with no
inflammatory cell infiltrates and reduction of
myeloperoxidase (MPO) activities when compared with
colitis control group. AVE pretreatment ameliorated
significantly the clinical and histopathological
severity of the TNBS-induced colitis; decreased body
weight loss and diarrhea and increased survival.
Conclusion: It was concluded that oral
administration of AVE represents a valuable
therapeutic approach for the treatment of colitis in
chicken.
Keywords: Aloe vera, Colitis, TNBS,
Chicken, Macrophages, Enzyme Assay.
