Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Original Research
5.
Isolation and prevalence of Mycoplasma agalactiae in
Kurdish sheep in Kurdistan, Iran -
Mohammad Khezri, Seyed Ali Pourbakhsh, Abbass
Ashtari, Babak Rokhzad, Homan Khanbabaie
Vet World. 2012; 5(12): 727-731
doi:
10.5455/vetworld.2012.72
7-731
Abstract
Aim:
Ruminant Mycoplasmosis are important diseases
worldwide and several are listed by the World
Organization for Animal Health (OIE) to be of major
economic significant. The aim of this study was to
isolation mycoplasmas from sheep presenting
contagious agalactiae (CA) in Kurdistan in the West
of Iran.
Materials and Methods: Sixty-nine
samples included (milk, conjuctiva swabs, synovial
fluid and ear canal swabs) were examined by PCR
assay during 2011-2012. DNA was extracted from
enriched samples. Two primers (forward and reverse)
amplify a 163bp region of 16S rRNA gene of
Mycoplasma genus and two primers amplify 375bp
region of 16S rRNA gene of Mycoplasma agalactiae (M.
agalactiae) species were used.
Results: This
proved that 46 samples (66.7%) were infected with
Mycoplasma in culture and PCR test, respectively. On
the PCR test, 15 isolates (32.6%) examined were
positive for M. agalactiae that showed specific
amplicon at 375bp. All Mycoplasma positive samples
were analyzed for M. agalactiae infection by PCR
method and 31 isolates (67.4%) examined were
negative for M. agalactiae. The finding of other
mycoplasmas with significant epidemiology challenges
existing plans for the control of CA in sheep
population in Iran. Conclusion: The results
of the present study show that M. agalactiae in CA
disease in Kurdistan Province, 32.6% involved. In
Iran, only M. agalactiae vaccines are commercially
available, thus, the animals are not protected
against these other species.
Keywords:
Contagious agalactiae, Iran, Mycoplasma agalactiae,
PCR
