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Open Access
This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Original Research
8. Preservation
of washed spermatozoa of mehsana buck at
refrigeration temperature -
V R Parmar, B N Suthar, V K Sharma, H C Nakhashi
and
S S Parikh
Vet World. 2012; 5(5): 294-296
doi:
10.5455/vetworld.201 2.294-296
Abstract
The study was done to understand the preservation
of Washed Spermatozoa of Mehsana Buck at
Refrigeration temperature. The washed spermatozoa
from 78 semen ejaculates, 26 each from 3 Mehsana
bucks, were preserved at 4 ± 10C temperature in SM
and TCFEY diluents up to 72 hours and the physical
characteristics of spermatozoa were studied to
assess the suitability of diluents. The individual
sperm motility and live spermatozoa decreased
significantly whereas abnormal spermatozoa
increased significantly (P<0.05) at each 12 hrs
interval of preservation in two diluents. The
effect of diluents on spermatozoan characteristics
was less pronounced in SM than TCFEY diluent.
Among the interaction studies, stage of
preservation affected the live spermatozoa and
their morphology. The findings suggested
superiority of SM diluent over TCFEY for
preservation of Mehsana buck semen.
Key words:
Buck ,Diluents, Ejeculates, Semen
Preservation, Spermatozoa.
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