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Original Research
2.
Biotyping of Bacillus cereus from the street vended
foods in Srinagar area of Kashmir -
Yasir Hafeez, Asif Iqbal, Manzoor Ahmad
Vet World. 2012; 5(10): 590-593
doi:
10.5455/vetworld.2012.5
90-593
Abstract
Aim:
The present study was undertaken to describe the
biotyping of Bacillus cereus isolated from different
street vended mutton tikka and chutney samples.
Materials and Methods: A total of 100 street
vended food samples comprising of 60 mutton tikka
and 40 chutney samples were tested.
Results:
The biotype 3 and biotype 4 showed the highest
occurrence with, 29.63% and 25.93% isolates falling
in these biotypes, respectively. The percentage
occurrence of the biotypes 1, 6, 2, 5, and 7 was
14.81%, 11.11%, 7.40%, 7.40% and 3.84%,
respectively. The most common found biotypes in
Mutton tikka were biotypes 3(29.63%), 4(25.93%),
1(14.81%) and 6(11.11%). The Bacillus cereus strains
isolated from chutney samples could be divided into
7 of the 9 possible biotypes. The biotypes 6 and 7
showed the highest occurrence with 38.46% and 30.76%
falling in these biotypes, respectively. The biotype
5 and 2 were prevalent to the extent of 23.07%,
7.69%, respectively. The biotypes 3, 4 and 1 were
absent. The mean bacterial count of 60 mutton tikka
and 40 chutney samples was 4.6817 and 5.6575 log10
cfu/g.
Conclusion: The field isolates and the
standard strains of Bacillus cereus had similar
cultural, morphological and biochemical
characteristics. The biotypes recovered from the
Mutton tikka samples were biotypes 3, 4, 1 and 6 and
in chutneys the biotypes recovered were 6, 7, 5 and
2. The strains of Bacillus cereus were highly
resistant to penicillin G (92.59%).
Keywords:
Bacillus cereus, biotyping, chutney, Mutton tikka
