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Original Research
6.
In vitro evaluation of salinomycin addition in wheat
straw based total mixed diets on rumen fermentation,
methanogenesis and dry matter degradability in
buffalo -
Sunil K. Sirohi, Navneet Goel
Vet World. 2012; 5(10): 609-613
doi:
10.5455/vetworld.2012.
609-613
Abstract
Aim:
The aim of the current study was to evaluate the
effect of salinomycin in vitro on methanogenesis and
rumen fermentation.
Materials and Methods:
Different levels of (0,10, 15 and 20 ppm)
salinomycin were checked for their effect on in
vitro methanogenesis and rumen fermentation on three
wheat straw based diets i.e. low fiber diet (LFD,
40R:60C), medium fiber diet (MFD, 50R:50C) and high
fiber diet (HFD, 60R:40C). Evaluation of salinomycin
was carried out using in vitro gas production
technique. Methane production and individual fatty
acids were estimated by Gas Chromatography.
Results: Results of different levels of
salinomycin on in vitro methanogenesis indicated
that the maximum methane reduction (38.14% in term
of mM/gDM) was noticed in HFD at 20 ppm level. IVDMD
showing increasing trend with an increasing
concentration of salinomycin with HFD and LFD, while
shown decreasing trend with MFD respectively.
Protozoal population significantly decreased by
addition of salinomycin in all diets. Conclusion:
The results of salinomycin evaluation in the current
study can be implicated to mitigate the methane
production, thus saving the feed energy loss and the
accumulation of green house gases in environment.
Keywords: In vitro gas production technique,
IVDMD, Methanogenesis, Salinomycin
