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Original Research
2.
Detection of Escherichia coli from the udder of the
dairy farm buffaloes in Phagwara region, Punjab,
India - Rajdeep Palaha, Narendra Chaudhary, Harsh Kumar
Vet World. 2012; 5(9): 522-525
doi:
10.5455/vetworld.2012.5
22-525
Abstract
Aim:
To know the presence of Escherichia coli on the
udder skin of the dairy farm buffaloes in the
Phagwara region, Punjab, India.
Materials and
Methods: A total of 135 swabbed samples were
collected randomly from the udder of buffaloes in
ten dairy farms over the period of three months from
August to October 2011 without concern to their
breed with the prior approval of the farm owners.
The sterilized cotton swabs were examined by Gram's
staining for the morphology of the culture, culture
characteristics was confirmed by growth on different
media and by preforming the different biochemical
tests like Indole production, Voges- Proskauer test,
Urease Production, Nitrate Reduction, Methyl red and
Presumptive test.
Results: Out of 135 samples
were examined, 23(17.03%) were positive for E. coli.
Most Probable Number (MPN) results confirmed the one
possibility of the bacteria from the contaminated
water.
Conclusion: The results of the present
study suggest that E. coli isolates are present on
the udder skin of the dairy farm buffaloes in the
Phagwara region, pose a serious threat to the animal
as well as consumer health. Thus, more hygienic
preventive measures are required to inhibit the
bacterial growth, so as to improve the health of the
animals as well as the wholesomeness of the milk.
Keywords: Buffaloes, Escherichia coli, Milk,
Mastitis, Phagwara, Udder.
