Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Original Research
3.
Sex determination of cattle meat by polymerase chain
reaction amplification of the amelogenin (AMELX/AMELY)
gene - P. Gokulakrishnan, R. R. Kumar, B. D. Sharma, S. K.
Mendiratta, D. Sharma , O. P. Malav
Vet World. 2012; 5(9): 526-529
doi:
10.5455/vetworld.2012.5
26-529
Abstract
Aim:
The objective of this study was to develop a
simplified, efficient, and accurate protocol for
sexing of cattle meat based on the amelogenin (AMELX/AMELY)
gene using PCR technique which is superior to
earlier work in terms of band patterns.
Materials
and Methods: Based on the amelogenin gene
located on the conservation region of X and Y
chromosomes, a pair of primers was designed and the
system of PCR was established to amplify a 241-bp
fragment from the X chromosome in female cattle, and
a 241-bp fragment from X chromosome and 178-bp
fragment from the Y chromosome in male cattle,
respectively. The accuracy and specificity of the
primers was assessed using DNA template extracted
from cattle meat samples of known sex. The protocol
was subjected to a blind test showed 100%
concordance, proving its accuracy and reliability.
Results: PCR products of cattle meat samples
after electrophoresis showed two bands (241, 178-bp)
for tissue from male while female tissue resulted in
only one (241-bp) band.
Conclusions: Our
findings show that the PCR assay based on the
amelogenin gene is reliable for sex determination in
cattle meat.
Keywords: Amelogenin gene,
Cattle meat, PCR, Sex determination
