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Original Research
5.
Microscopic changes due to fumonisin B and
ochratoxin A 1 induced nephropathy in Japanese quail -
Manzoor Ahmad Khan, Asif Iqbal , Rajesh Kumar Asrani
Vet World. 2012; 5(9): 535-540
doi:
10.5455/vetworld.2012.5
35-540
Abstract
Aim:
The present study was designed to evaluate the toxic
interaction of fumonisin B1 and ochratoxin A, alone
and in combination for pathology of the kidneys in
day old Japanese quail.
Materials and Methods:
Day old Japanese quail chicks were divided into
4 groups and fed with basal quail chick mash
containing fumonisin B1 @ 200 ppm (Group FX),
Ochratoxin A @ 2 ppm (Group OX), and fumonisin B1 @
200 ppm + Ochratoxin A @ 2 ppm (Group FO) and
standard toxin free feed (Group CX, control) for 28
days.
Results: The quail chicks were assessed
for various microscopic changes in the kidneys. Mean
microscopic score values for congestion was found to
be significantly higher (P< 0.05) in FO group when
compared to that of other groups FX and OX at 7 and
21 DPF. Similarly, mean microscopic score values for
degeneration was found to be significantly higher (P
≤ 0.05) in FO group as compared to that of groups FX
and OX at 7 and 14 DPF. Mean microscopic score
values for necrosis and luminal hyaline bodies were
significantly higher (P ≤ 0.05) in FO group when
compared to that of groups OX and FX at 7 DPF.
Conclusion: Total score card for microscopic
lesions observed in the kidneys when compared
between different treatment groups showed that the
lesions appeared to be more pronounced in the OTA
fed group at 14 DPF as compared to other treatment
groups. The overall lesion score values after the
conclusion of the experiment were found to be higher
in the combination group followed by the groups OX
and FX, respectively.
Keywords: Fumonisin B1,
ochratoxin A, Japanese quail, total lesion score and
mean lesion score.
