Aim: To
clone the virulent gene LipL32 of Leptospira interrogans serovar Autumnalis and
to analyze the sequence with LipL32 gene of other pathogenic serovars of
Leptopsira.
Materials and Methods: Leptospira interrogans serovar
Autumnalis procured from Leptospira research laboratory, Chennai was used in the
study. Polymerase chain reaction (PCR) was carried out for amplifying LipL32
gene using the reported primers of Leptospira Kirschnerii. The PCR product was
cloned into TA cloning vector and the vector was transformed into E.Coli DH5α
cells. The plasmid was isolated from E.Coli and sent for sequencing with
universal primers. The sequence was submitted in genbank with accession number
JQ861883. Results: The PCR product revealed an amplicon of 790 bp. The
LipL32 gene sequence of Leptospira interrogans serovar Autumnalis showed 99 %
similarity with most of the pathogenic Leptospires.
Conclusions: LipL32
gene of Leptospira is highly conserved in most of the pathogenic Leptospires.
The study concludes that this gene could be used as a target for the diagnosis
of leptospirosis in animals and humans and could be tested as an important
candidate antigen for vaccine production.
Keywords: Cloning, Sequencing,
LipL32 gene, Leptospira Autumnalis
