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Research
1. Experimental study on the effect of vitamin C
administration on lipid peroxidation and antioxidant enzyme activity in rats
exposed to chlorpyriphos and lead acetate -
Nisar Ahmad Nisar, Mudasir Sultana, Hina Ashraf Waiz, Parveez Ahmad Para,
Naseer Ahmad Baba, Fayaz Ahmad Zargar and Waseem Hussain Raja
Vet World. 2013; 6(8): 461-466
doi:
10.5455/vetworld.2013.461-466
Abstract
Aim: To evaluate the effects of
chlorpyriphos, lead acetate, vitamin C alone, and in combination on the
activity of oxidative stress parameters in wistar rats.
Materials and
Methods: Rats of 150-200g body weight were divided into eight groups of
six animals each and were subjected to various daily oral treatment regimes
for 98 days. Group I served as control receiving only corn oil, group II
received chlorpyriphos @ 5.5 mg/ kg in corn oil, group III received lead
acetate @100 ppm in water, whereas animals in group IVth received a
combination of chlorpyriphos @ 5.5mg/kg in corn oil and lead acetate @ 100 ppm
in water. Group Vth received vitamin C @ 100mg/kg in water, group VIth
received a combination of chlorpyriphos @ 5.5mg/kg and vitamin C @ 100mg/kg ,
group VIIth received lead acetate @ 100 ppm in water and vitamin C @ 100mg/kg
and group VIIIth received chlorpyriphos @ 5.5mg/kg , lead acetate @100ppm in
water and vitamin C @ 100mg/kg.
Results: Administration of both
chlorpyriphos and lead acetate caused a significant decrease in oxidative
stress parameters viz. blood glutathione, catalase, superoxide dismutase
(SOD), glutathione peroxidase (GPx), glutathione-s-transferase (GST) along
with a significant increase in lipid peroxidation level when given alone or in
combination.
Conclusions: The study demonstrated that treatment of
chlorpyriphos and lead treated rats with vitamin C significantly improved some
of altered oxidative stress parameters revealing the protective effect of this
vitamin C against oxidative stress induced by chlorpyriphos and lead.
Keywords: chlorpyriphos, lead acetate, oxidative stress, vitamin C
