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Research
(Published
online : 24-05-2013)
15. Two-stage in vitro digestibility assay, a
tool for formulating non-starch polysaccharide degrading enzyme combinations
for commonly used feed ingredients of poultry rations -
J. Narasimha, D. Nagalakshmi, Y. Ramana Reddy and S. T. Viroji Rao
Vet World. 2013; 6(8): 525-529
doi:
10.5455/vetworld.2013.525-529
Abstract
Aim: An attempt was made to
assess the effect of pure enzyme combinations with the objective of
formulating customized enzyme mixtures based on sugar release when subjected
to two-stage in vitro digestion assay. Materials and Methods: A
two-stage in vitro digestibility assay was carried out for commonly used feed
ingredients for poultry viz., maize, soy bean meal, sunflower cake, and
de-oiled rice bran supplemented with three concentrations of xylanase (5000;
7500 and 10000 IU/kg), cellulase (50; 100 and 400 IU/kg) and β-D-glucanase
(100; 200 and 400 IU/kg) were used to formulate various NSP enzymes
combinations. In total 27 NSP enzyme combinations (3x3x3) were formulated and
the sugar released due to NSP digestion was quantified by phenol sulphuric
acid method.
Results: The total sugar release was significantly
(P<0.05) higher with supplementation of various enzymes combinations for
maize, sunflower cake and de-oiled rice bran where as no significant (P<0.05)
interaction of various NSP enzymes combinations was observed for soy bean
meal. The NSP digestibility was highest in combination (xylanase-5000,
cellulase-50 and β-D-glucanase-400 IU/kg), (xylanase-10000, cellulase-50 and
β-D-glucanase-200 IU/kg) and (xylanase-7500, cellulase-100 and
β-D-glucanase-100 IU/kg) for maize, sunflower cake and de-oiled rice bran
respectively. In case of sunflower cake, significant (P<0.01) three way
interaction was observed among the xylanase, cellulose, and β-D-glucanase
enzymes and the two-way interactions between the enzymes were also significant
(P<0.01).
Conclusion: It is concluded that 'n' number of non-starch
Polysaccharide enzymes combinations can be screened for their efficiency to
digest non-starch Polysaccharides present in various feed ingredients commonly
used in poultry rations by employing two-stage in vitro digestibility assay as
a tool.
Keywords: feed ingredients, in vitro digestibility assay,
non-starch polysaccharide enzymes
