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              Research 
(Published online : 01-06-2013)
20. Antibiogram of 
bacterial species isolated from canine pyometra -
Varun Bassessar, Yamini Verma and 
Madhu Swamy
Vet World. 2013; 6(8): 546-549
              
              
               
              
              doi: 
              10.5455/vetworld.2013.546-549
               
              
  
  
   
  
              
               
               
               
              
              
              Abstract
              
              
              
Aim: The aim of the present work was to ascertain the bacterial flora 
causing pyometra in female dogs and their antibiotic sensitivity. 
              
              
              Materials 
and Methods: A study was conducted to determine the antibiogram of bacterial 
species isolated from 20 female dogs diagnosed with pyometra. The vaginal 
discharge was collected by sterile swab and streaked smoothly over Mueller 
Hinton medium and sensitivity towards antibiotics was determined by measuring 
the zone of inhibition using a Hi-media scale. 
              
              
              Results: The antobiogram 
showed that Gentamicin was the most sensitive (85%) antibiotic followed by 
Enrofloxacin, Ciprofloxacin and Amoxicillin (65%, 65% and 55%), respectively. 
The isolates were most resistant to Oxytetracycline (85%) followed by 
Tetracycline, Ampicillin, Chloramphenicol, Cloxacillin and Erythromycin (80%, 
80%, 75%, 70% and 70%), respectively. 
              
              
              Conclusion: Gentamicin was found to 
be most effective antibiotic against the bacterial species isolated from canine 
pyometra. 
              
              
              Keywords: antibiotic, antibiogram, canine, pyometra, 
sensitivity test