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Research
(Published
online : 13-06-2013)
24. Repeated
dose oral toxicity of inorganic mercury in wistar rats: biochemical and
morphological alterations - T. J. Sheikh, B. J. Patel, D. V. Joshi,
R. B. Patel and M. D. Jegoda
Vet World. 2013; 6(8): 563-567
doi:
10.5455/vetworld.2013.563-567
Abstract
Aim: The study was conducted
to find out the possible toxic effect of mercuric chloride (HgCl2 ) at the
histological, biochemical, and haematological levels in the wistar rats for 28
days.
Materials and Methods: The biochemical and hematological alteration
were estimated in four groups of rat (each group contain ten animals), which
were treated with 0 (control), 2, 4, and 8 mg/kg body weight of HgCl2 through
oral gavage. At the end of study all rats were sacrificed and subjected for
histopathology.
Result: A significantly (P < 0.05) higher level of serum
alanine amino transferase (ALT), gamma Glutamyle Transferase, and creatinine
were recorded in treatment groups, while the level of alkaline phosphtase (ALP)
was significantly decreased as compared to the control group. The toxic effect
on hematoclogical parameter was characterized by significant decrease in
hemoglobin, packed cell volume, total erythrocytes count, and total leukocyte
count. Gross morphological changes include congestion, severe haemorrhage,
necrosis, degenerative changes in kidneys, depletion of lymphocyte in spleen,
decrease in concentration of mature spermatocyte, and edema in testis. It was
notable that kidney was the most affected organ.
Conclusion: Mercuric
chloride (HgCl2 ) caused dose-dependent toxic effects on blood parameters and
kidney.
Keywords: biochemical, haematological, mercuric chloride,
morphological, toxicity, wistar rat
