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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published online : 14-06-2013)
27. Sperm selection techniques and antioxidant fortification in low grade
semen of bulls: Review -
Thakur Krishna Shankar Rao, Niranjan Kumar,
Navinkumar B. Patel, Indrasen Chauhan and Shailendra Chaurasia
Vet World. 2013; 6(8): 579-585
doi:
10.5455/vetworld.2013.579-585
Abstract
The low grade
ejaculates are very common in bulls. Low grade ejaculates might be due to age
or non specific factors like thermal stress, transport and vaccination stress
during the dynamic life of bulls. Lipid peroxidation of membrane induced sperm
damage further aggravates the situation. Researches reveal that selection of
sperm and antioxidant fortification play crucial role in improving the quality
of semen. Different methods used for semen up-gradation like washing,
sedimentation, swim up procedure and filtration like percoll gradient, glass
wool, sephadex and sephadex ion exchanger with significant improvement in
motility, Hypo osmotic swelling test reactivity, viability and acrosomal
integrity. Antioxidants are added directly to extender at standard dose rate
with positive result. Among different filtration column used sephadex ion
exchanger (FS+IE) was superior over other in improving the semen quality
especially when fortified with Vitamin E. Moreover a complete protocol is
required, which contain both antioxidant fortification and sperm selection
simultaneously to handle the low grade semen from sub-fertile bulls.
Keywords: antioxidant, bull, genetic, semen, sperm
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