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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online : 01-12-2013)
2. Preliminary acute toxicity study on imidacloprid in
Swiss albino mice - Preeti Bagri, Vinod Kumar, Anil Kumar Sikka and
Joginder Singh Punia
Veterinary World, 6(12): 955-959
doi:
10.14202/vetworld.2013.955-959
Abstract
Aim: To ascertain the maximum
tolerated dose (MTD) and to investigate the acute oral toxic
effects of imidacloprid towards Swiss albino male mice.
Materials and Methods: The MTD of imidacloprid was
determined in pilot dose range finding study following the
standard method. Animals were observed for toxic signs and
symptoms after oral administration of MTD of imidacloprid in
single dose. The body weights of animals were recorded on
alternate day. Animals were sacrificed on 14th day and changes in
hematological parameters (Hb, TEC, TLC and DLC) and morphometric
measurements (length, breadth, thickness and weight) of various
body organs (heart, liver, spleen, kidney, testis and epididymis)
were examined. The student's t-test was applied to statistically
analyze the results.
Results: The MTD of imidacloprid was determined to be 110
mg/kg body weight. The sign and symptoms of acute toxicity were
ataxia, rigidity and fasciculation of muscles, protrusion of eye
ball and tremors of head. Imidacloprid treatment resulted in
decreased body weight gain as compared to the control group. The
changes in hematological parameters were not significant between
imidacloprid treated and control groups. Also the values of
relative organ weights and morphometric measurements of various
body organs did not differ significantly between the control and
imidacloprid treated animals.
Conclusions: MTD of imidacloprid in Swiss albino male mice
through oral route was determined for the first time. Study
revealed a non-toxic effect of imidacloprid on body weight,
relative organs weight, hematological parameters and morphometric
measurements of various body organs in mice.
Keywords: hematology, imidacloprid, morphometric
measurement, MTD, toxicity
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