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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online: 13-12-2013)
7. Differential expression of prostaglandin F receptor
in goat endometrium during estrous cycle and early pregnancy - Rohit
Kumar, P.W. Ramteke, Amarnath and Sanjeev Kumar Sharma
Veterinary World, 6(12): 978-981
doi:
10.14202/vetworld.2013.978-981
Abstract
Aim: Present study was
undertaken to investigate profiling of prostaglandin F receptor (PGFR)
mRNA expression during estrous cycle and early pregnancy.
Materials and Methods: Female genitalia of goats (n=3) were
collected from a local abattoir. Based on color, vasculature,
size, and consistency of corpus luteum (CL), the uteri were
classified into three stages of the estrous cycle: stage I: Days
3–5; stage II: Days 6–15, stage III: Days 16–21 and early
pregnancy (<30 d foetus). Total RNA was isolated from endometrial
scrapings of respective uteri and reverse transcribed followed by
DNase treatment. cDNA was used as template to assess gene
expression. Expression profiling was performed for PGFR gene using
Real Time quantitative PCR (RT-qPCR).
Results: At the time of luteolysis the expresion of PGFR
was found to be high. However, during pregnancy the expression of
PGFR was high as compared to stages of estrous cycle.
Conclusion: Higher expression of PGFR at stage III showed
luteolytic signal during estrous cycle. However, peaked expression
during pregnancy revealed that the conceptus could be responsible
for upregulated expression of PGFR in the endometrium during the
implantation period.
Key words: early pregnancy, goat, estrus cycle,
prostaglandin F Receptor, prostaglandin F2 α
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