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              Open Access  
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, 
distribution and reproduction in any medium, provided the work is properly 
cited. 
 
                              
                              
                              Review Article 
                              
                              
                              15.  Faecal steroid 
                              metabolites assay as a non-invasive monitoring of 
                              reproductive status in animals - Ashok 
                              Kumar, S Mehrotra, S S Dangi, G Singh, Subhash 
                              chand, Lakhanpal Singh, A S Mahla, Sachin Kumar 
                              and  K NehraVet World. 2013; 6(1): 59-63
 
                
              doi: 
              10.5455/vetworld.2013.59-63 
                
              
              
          
 
              Abstract 
 Measurement of faecal steroids as a non-invasive 
                              technique is widely used to monitor reproductive 
                              hormones in captive and free-ranging wild animals. 
                              This method offers a great advantage over invasive 
                              techniques like blood collection and deserves to 
                              be used in domestic animals. Repeated blood 
                              sampling is stressful, having animal welfare 
                              issues, difficult to obtain in field condition. In 
                              the faeces of cow, estradiol 17-α predominated, 
                              whereas in mares and sows, estradiol 17-β and 
                              estrone are main estrogens. Steroids are 
                              metabolized in liver, excreted in faeces and 
                              extraction is done by using a variety of methods 
                              such as ethanol, methanol etc. Faecal estrogen and 
                              progesterone metabolites evaluations are wel1 
                              established approaches for monitoring of 
                              reproductive functions in a variety of mammalian 
                              species. So in future this method may be the most 
                              suitable for monitoring of reproductive status in 
                              farm animals particularly under field conditions.
               
                              Keywords: Faecal steroids, non-invasive 
                              technique, estrogen, progesterone metabolites, 
                              reproductive status 
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