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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Original
Research
3. Isolation and
identification of Staphylococcus aureus
from milk and milk products and their drug
resistance patterns in Anand, Gujarat -
H. C. Thaker, M. N. Brahmbhatt and J. B. Nayak
Vet World. 2013; 6(1): 10-13
doi:
10.5455/vetworld.201 3.10-13
Abstract
Aim: The study was carried out with aim to
isolate Staphylococcus aureus from milk and milk
products (pedha and curd) and determine
antibiogram pattern of S. aureus isolates.
Materials and Methods: During 9 months
duration of study a total of 160 milk and milk
product samples (pedha and curd) were collected
from different places in and around Anand city
such as milk collection centre of Co-operative
milk dairies, cattle farms, individual household,
milk vendors and sweet shops. The samples were
collected under aseptic precautions and were
enriched in Peptone Water (PW) followed by direct
plating on selective media viz. Baird-Parker Agar.
The presumptive S. aureus isolates were identified
by biochemical tests. Antibiogram pattern of S.
aureus to antimicrobial agents were evaluated by
disk diffusion method.
Results: Analysis of
result revealed that out of total 160 samples of
milk (100) and milk products i.e. curd (30) and
pedha (30) resulted in the isolation of 10
isolates (6.25%) of S. aureus. In the present
study S. aureus isolates were found variably
resistant to the antibiotics tested. The S. aureus
isolates showed highest sensitivity towards
cephalothin (100.00%), co-trimoxazole (100.00%),
cephalexin (100.00%) and methicillin (100.00%)
followed by gentamicin (90.00%), ciprofloxacin
(80.00%), oxacillin (70.00%), streptomycin
(60.00%) and ampicillin (60.00%). The pattern
clearly indicated that the overall high percent of
S. aureus isolates were resistant to Penicillin-G
(100.00%) followed by ampicillin (40.00%),
oxytetracycline and oxacillin (20.00%) and
streptomycin and gentamicin (10.00%).
Conclusions: Results clearly suggested a
possibility of potential public health threat of
S. aureus resulting from contamination of milk and
milk products with pathogenic bacteria is mainly
due to unhygienic processing, handling and
unhygienic environment.
Key words:
antibiogram pattern, milk products, S. aureus,
staphylococcal food poisoning
References
1. Loir, Y.L., Baron, F. and Gautier, M. (2003). Staphylococcus
aureus and food poisoning. Gen. & Mol. Res., 2(1): 63-76.
[Pubmed]
2. Soomro, A.H., Arain, M.A., Khaskheli, M. and Bhutto, B. (2003).
Isolation of Staphylococcus aureus from milk products sold at
sweet meat shops of Hyderabad. Online J. Biol. Sci., 3(1): 91-94.
3. Al-khatib, I.A. and Al-Mitwalli, S.M. (2009). Microbiological
quality and sample collection policy for dairy products in
Ramallah and Al-Bireh district, Palestine. East Mediterranean
Heal. J., 15: 709-716.
4. Ismael, Z.S., Hosny, W.I., El-Kholy, W.I. and El-Dairouty, R.K.
(2009). Comparative investigations for detection of foodborne
microorganisms in Egyptian hard cheese (Ras): using conventional
and fast biochemical test. J. Global Vet.,3: 189-195.
5. Chapaval, L., Moon, D.H., Gomes, J.E., Duarte, F.R. and Tsai,
S.M. (2010). Efeito da temperatura sobre a produção de
enterotoxina estafilocócica em leite. Revista Higiene Alimentar.,
24: 180-181.
6. Balaban, N. and Rasooly, A. (2000). Staphylococcal enterotoxins.
Int. J. Food Microbiol., 61: 1-10.
[DOI
via Crossref]
7. Presscott, L.M., Harley, J.P. and Klein, D.A. (2002). Text book
of Microbiology. Brown Publishers. 5th ed.: 441-442.
8. Bergdoll, M.S. (1983). Enterotoxins. Staphylococci and
Staphylococcal Infections (Easman, C.S.F. and Adlam, C., eds.).
Academic Press, London, UK.: 559-598.
9. Payne, D.N. and Wood, J.M. (1974). The incidence of enterotoxin
production in strains of Staphylococcus aureus isolated from food.
J. Appl. Bacteriol., 37(3): 319-325.
[DOI
via Crossref]
[Pubmed]
10. Bhatia, A. and Zahoor, S. (2007). Staphylococcus aureus
Enterotoxins: A Review. J. Clin. & Diag. Res., 1: 188-197.
11. Haluk, C., Leyla, V. and Nebahat, B.O. (2010). Isolation of
Staphylococci from Food Handlers and Investigation of Their
Enterotoxigenicity and Susceptibility to Some Antibiotics. J.
Faculty vet. Med., University of kafkas. 16: S1-S5.
12. Singh, P. and Prakash, A. (2008). Isolation of Escherichia
coli, Staphylococcus aureus and Listeria monocytogenes from milk
products sold under market conditions at Agra Region. Acta Agri.
Slov., 92(1): 83-88.
13. Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turck,M. (1966).
Antibiotic susceptibility testing by a standardized single disk
method. Amer. J. Clin. Pathol., 45: 493-496.
[Pubmed]
14. Holm, C. and Jespersen, L. (2003). A flow-cytometric Gram-Stainung
technique for milk associated bacteria. Appl. and Environ.
Microbiol., 69: 2857-2863.
[DOI
via Crossref]
[Pubmed]
[PMC
Free Fulltext]
15. Vasavada, P.C. (1988). Pathogenic bacteria in milk-A review.
J. Dairy Sci., 71: 2809-2816.
[DOI
via Crossref]
16. Bonfoh, B., Wasem, A., Traore, A.N., Fane, A., Spillmann,
H.,Simbe, C.F., Alfaroukh, I.O., Nicolet, J., Farah, Z. and
Zinsstag, J. (2003). Microbiological quality of cow's milk taken
at different intervals from the udder to the selling point in
Bamako (Mali). Food Control, 14: 495-500.
[DOI
via Crossref]
17. Altekruse, S., Hyman, F., Klontz, K., Timbo, B. and Tollefson,
L. (1994). Food borne bacterial infections in individuals with the
human immunodeficiency virus. South Med. J., 87: 69-73.
[DOI
via Crossref]
18. Fagundes, H., Barchesi, L., Filho, A.N., Ferreira, L.M. and
Oliveira, C.A.F. (2010). Occurrence of Staphylococcus aureus in
raw milk produced in dairy farms in São Paulo State, Brazil. Braz.
J. Microbiol., 41: 376-380.
[DOI
via Crossref]
19. Shah, N.M., Kher, H.N., Dholakia, P.M. and Simaria, M.B.
(1985). Studies on Staphylococci in udder of cattle. Indian Vet.
J., 62: 458-460.
20. Kumar, R. and Prasad, A. (2010). Detection of E.coli and
Staphylococcus in Milk and Milk Products in and around Pantnagar.
Vet. World., 3(11): 495-496.
21. Tambekar, D.H. and Bhutda, S.A. (2010). Prevalence of
Bacteriological pathogens in Pedha (a milk product) sold in
Amrawati (India). Int. J. Dairy Sci., 5(3): 173-176.
[DOI
via Crossref]
22. Ekici, K., Bozkurt, H. and Isleyici, O. (2004). Isolation of
Some Pathogens from Raw Milk of Different Milch Animals. Pak J.
Nutri., 3(3): 161-162.
[DOI
via Crossref]
23. Santana, E.H.W., Cunha, M.L.R.S., Oliveira, T.C.R.M., Moraes,
L.B. and Alegro, L.C.A. (2010). Assessment of the risk of raw milk
consumption related to staphylococcal food poisoing. Ciência
Animal Brasileira., 11: 643-652.
24. Zakary, E.M., Nassif, M.Z. and Mohammed, G.M.O. (2011).
Detection of Staphylococcus aureus in Bovine Milk and its Product
by Real Time PCR Assay. Global J. Biotech. & Biochem., 6(4):
171-177.
25. Lingathurai, S. and Vellathurai, P. (2011). Bacteriological
quality and safety of raw cow milk in Madurai, South India. Webmed
Cent. Microbiol., 1: 1-10.
26. Normanno, G., La Salandra, G., Dambrosio, A., Quaglia, N.C.,
Corrente, M., Parisi, A., Santagada, G., Firinu, A., Crisetti, E.
and Celano, G.V. (2007). Occurrence, characterization and
antimicrobial resistance of enterotoxigenic Staphylococcus aureus
isolated from meat and dairy products. Int. J. Food Microbiol.,
115: 290–296.
[DOI
via Crossref]
[Pubmed]
27. Addis, M., Pal, M. and Kyule, N. (2011). Isolation and
Identification of Staphylococcus species from Raw Bovine Milk in
Debre Zeit, Ethiopia. Vet. Res., 4(2): 45-49.
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