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Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Original Research (Published online : 16-03-2013)

14. Prevalence of Bovine Herpesvirus-1 in cattle and buffaloes in Punjab - Gurpreet Kaur, P. N. Dwivedi, Ramneek Verma and N. S. Sharma
Vet World. 2013; 6(6): 343-345

 

doi: 10.5455/vetworld.2013.343-345

 

 

 

 

Abstract

Aim: The aim of the present study was to identify the prevalence of Bovine Herpesvirus-1 (BHV-1) in cattle and buffaloes in the Punjab using PCR as diagnostic tool.

Materials and Methods: A total of 63 samples (Semen- 57, placental cotyledons-1, vaginal secretions-1, foetal stomach contents-1 and tracheal swabs-3) from cattle and buffaloes were processed for identification of BHV-1 using PCR.

Results: From January 2007 to December 2010 (Semen- 57, placental cotyledons-1, vaginal secretions-1, foetal stomach contents-1 and tracheal swabs-3) from cattle and buffaloes were collected. The DNA was extracted from a total of 63 samples and subjected to PCR revealed that none of the sample positive for the BHV-1 infection.

Conclusion: From the study it was concluded that the farms screened were free from BHV-1 infection. Keywords: Bovine Herpesvirus-1, buffaloes, cattle, PCR, prevalence