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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 15-10-2013)
13. Genetic characterization of complete open reading
frame of glycoprotein C gene of bovine herpesvirus 1 - Saurabh
Majumder, A. B. Pandey and M. A. Ramakrishnan
Veterinary World, 6(11): 897-900
doi:
10.14202/vetworld.2013.897-900
Abstract
Aim: To characterize one of
the major glycoprotein genes viz., glycoprotein C (gC; UL44,
unique long region 44) of bovine herpesvirus 1(BoHV1) of Indian
origin at genetic and phylogenetic level.
Materials and Methods: A bovine herpesvirus 1 isolate viz.,
(BoHV1/IBR 216 II/ 1976/ India) maintained at Division of
Virology, IVRI, Mukteswar was used for the current study. The DNA
was extracted using commercial kit and the complete ORF of gC gene
was amplified, cloned, and sequenced by conventional Sanger
sequencing method. The sequence was genetically and
phylogenetically analysed using various bioinformatic tools. The
sequence was submitted in the Genbank with accession number
Kc756965.
Results: The complete ORF of gC gene was amplified and
sequenced. It showed 100% sequence homology with reference cooper
strain of BoHV1 and divergence varied from 0% to 2.7% with other
isolates of BoHV1. The isolate under study had divergence of 9.2%,
13%, 26.6%, and 9.2% with BoHV5 (Bovine herpesvirus 5), CvHV1 (Cervid
herpesvirus 1), CpHV1 (Caprine herpesvirus 1), and BuHV1 (Bubaline
herpesvirus 1), respectively.
Conclusion: This is the first genetic characterization of
complete open reading frame (ORF) of glycoprotein C gene (UL44) of
Indian isolate of BoHV1. The gC gene of BoHV1 is highly conserved
among all BoHV1 isolates and it can be used as a target for
designing diagnostic primers for the specific detection of BoHV1.
Key words: BoHV1, glycoprotein C, PCR, UL44.
References
1. Whetstone CA, Evermann JF (1988) Characterization of bovine
herpesviruses isolated from six sheep and four goats by
restriction endonuclease analysis and radioimmunopreci-
pitation. Am J Vet Res 49:781–785.
PMid:2840840 |
|
|
|
2. Qu XY, Li CH (1988) Studies on diagnosing infectious bovine
rhinotracheitis in yak by ELISA. China J Vet Sci Tech 3:5–8. |
|
|
3. Rajkhowa S, Rajkhowa C, Rahman H, Bujarbaruah KM (2004)
Seroprevalence of infectious bovine rhinotracheitis in mithun
(Bos frontalis) in India. Rev Sci Tech 23:821–829.
PMid:15861877 |
|
|
4. Nandi S, Kumar M, Manohar M, Chauhan RS (2009) Bovine
herpes virus infections in cattle. Anim Health Res Rev
10:85–98.
http://dx.doi.org/10.1017/S1466252309990028
PMid:19558751 |
|
|
5. Spiliki FR, Esteves PA, Lima MD, Franco AC, Chiminazzo C,
Flores EF, Weilblen R, Driemeier D, Roehe PM (2004)
Comparative pathogenecity of Bovine herpesvirus 1 (BHV- 1)
subtype 1 (BHV-1.1) and 2a (BHV-1.2a). Pesq Vet Bras 24:43–49.
http://dx.doi.org/10.1590/S0100-736X2004000100010 |
|
|
|
6. Anonymous (2005) The biology of bovine herpesvirus 1
(BoHV-1). Department of Health and Ageing, Office of the gene
technology regulator, Australian government 1–15. |
|
|
7. Traesel CK, e Silva MS, Spilki FR, Weiblen R, Flores EF
(2013) Nucleotide sequencing and phylogenetic analysis of the
3' region of glycoprotein C gene of South American bovine
herpesviruses 1 and 5. Res Vet Sci 94:178–185.
http://dx.doi.org/10.1016/j.rvsc.2012.07.032
PMid:22929314 |
|
|
8. Rijsewijk FA, Kaashoek MJ, Langeveld LD, Meloen R, Judek J,
Bienkowska-Szewczyk K, Maris-Veldhuis MA, Van orischot J T
(1999) Epitopes on glycoprotein C of bovine herpesvirus-1
(BHV-1) that allow differentiation between BHV-1.1 and BHV-1.2
strains. J Gen Virol 80:1477–1483.
PMid:10374966 |
|
|
|
9. Mehrotra ML, Rajya BS, Kumar S (1976) Infectious bovine
rhinotracheitis (IBR) – keratoconjunctivitis in calves. Indian
J Vet Pathol 1:70–73. |
|
|
10. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar
S (2011) MEGA5: molecular evolutionary genetics analysis using
maximum likelihood, evolutionary distance, and maximum
parsimony methods. Mol Biol Evol 28: 2731– 2739.
http://dx.doi.org/10.1093/molbev/msr121
PMid:21546353 PMCid:PMC3203626 |
|
|
11. Ros C, Belák S (1999) Studies of genetic relationships
between bovine, caprine, cervine, and rangiferine alpha
herpesviruses and improved molecular methods for virus
detection and identification. J Clin Microbiol 37:1247–1253.
PMid:10203465 PMCid:PMC84742 |
|
|
|
12. Gupta PK, Saini M, Rai A (2006) Rapid and sensitive PCR-
based test for detection of bovine herpesvirus-1 in semen.
Indian J virol 17:23–27. |
|
|
|
13. Nandi S, Kumar S (2011) Improved efficiency of the
polymerase chain reaction for detection of bovine
herpesvirus-1 genomic DNA. Buffalo bulletin 30: 250-255. |
|
|
14. Chowdhury SI (1995) Molecular basis of antigenic variation
between the glycoproteins C of respiratory bovine herpes virus
1 (BHV-1) and neurovirulent BHV-5. Virology 213: 558–568.
http://dx.doi.org/10.1016/S0042-6822(95)80137-5 |
|
|
15. Vigerust DJ, Shepherd VL (2007) Virus glycosylation: role
in virulence and immune interactions. Trends Microbiol
15:211–218.
http://dx.doi.org/10.1016/j.tim.2007.03.003
PMid:17398101 |
|
|
