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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 23-10-2013)
16. Detection of OmpA gene by PCR for specific
detection of Salmonella serovars - Joy. L. Kataria, A. Kumar, S.
Rajagunalan, L. Jonathan and R. K. Agarwal
Veterinary World, 6(11): 911-914
doi:
10.14202/vetworld.2013.911-914
Abstract
Aim: The study was carried
out to determine the sensitivity and specificity of OmpA gene in
Salmonella serovars through PCR.
Materials and Methods: A set of primers were designed
targeting the OmpA gene specific for the Salmonella and polymerase
chain reaction was standardized using Salomonella Typhimurium as a
positive control and as a negative control 4 non salmonella
cultures such as Campylobacter coli, Arcobacter butzleri, Brucella
abortus and E. coli. Sensitivity of the test was determined by
serial dilution of genomic DNA of standard S. Typhimurium. The PCR
standardized was used for screening 68 strains of different
serovars of Salmonella.
Results: The PCR developed targeting OmpA specific for
Salmonella was highly specific in detection of the salmonella
serovar alone and sensitivity was upto 68.8 fg. A total of 68
virulent/ natural strains of different serovars of salmonella
taken up for the study were positive by OmpA based PCR.
Conclusions: This study reports that, OmpA gene which is
conserved among Salmonella serovars can be used for the detection
of Salmonella in food or clinical samples in further studies, with
high sensitivity and specificity.
Key words: OmpA, PCR, Salmonella
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