|
|
Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 23-10-2013)
17. Occurrence of multidrug resistance shiga-toxin
producing Escherichia coli from milk and milk products - Javeed Ahmad
Sheikh, Mohd. Rashid, Majueeb U Rehman and M. A. Bhat
Veterinary World, 6(11): 915-918
doi:
10.14202/vetworld.2013.915-918
Abstract
Aim: The aim of this study
was to assess the hygienic quality of milk and milk products in
respect of shiga toxin producing Escherichia coli (STEC).
Materials and Methods: 205 samples of raw milk and milk
products were processed for isolation of E. coli. The isolates
were screened by mPCR for detection of virulence gene. 52 E. coli
isolates were tested against 15 commonly used antibiotics in the
field.
Results: Of the 205 samples of milk and milk products, 52
(25.36%) were positive for E. coli. Out of which 4% samples were
positive for STEC from raw milk and 3.64% from milk products.
Also, 25 of 52 (48%) of E. coli isolates were multidrug resistance
whereas 62.5% of STEC from milk and milk products were multidrug
resistance.
Conclusions: The milk and milk products production is of
poor hygienic quality in and around Jammu region which needs
improvement.
Key words: milk, mPCR, multidrug resistance, STEC
References
1. Solomakos, N., Govaris, A., Angelidis A. S. (2009)
Occurrence, virulence genes and antibiotic resistance of
Escherichia coli O157 isolated from raw bovine, caprine and
ovine milk in Greece. Food Microbiology, 26: 865–871.
http://dx.doi.org/10.1016/j.fm.2009.06.002
PMid:19835773 |
|
|
2. Armstrong, G.L., Hollingsworth, J. and Morris, J.G.J.
(1996) Emerging food borne pathogen: E. coli O157:H7 as a
model of entry of a new pathogen into the food supply of the
developed world. Epidemiology Review, 18: 29-51.
http://dx.doi.org/10.1093/oxfordjournals.epirev.a017914
PMid:8877329 |
|
|
|
3. Karch, H., Schmidt, H., Brunder, W. (1998) Plasmid- encoded
determinants in E.coli O157:H7.in:kaper, J.B., O, Brien, A.D.,
(Eds), E.Coli o157:H7 and other shiga toxin producing E.coli
strains. ASM press, Washington, D.C., pp.183-194. |
|
|
4. Griffin, P.M. and Tauxe. R.V. (1991)The epidemiology of
infections caused by Escherichia coli O157:H7, other
enterohaemorrhagic E. coli, and the associated hemolytic
uremic syndrome. Epidemiology Review, 13:60–98.
PMid:1765120 |
|
|
|
5. Griffin, P.M. (1995)Escherichia coli O157: H7 and other
hemorrhagic Escherichia coli. In infection of the
gastrointestinal tract, edited by M.J. Blaser, P.D. Smith, J.I.
Ravdin, H.B. Greenberg and R.I. Guerrant, pp. 739-761,New
York, N.Y.Raven Press, Ltd. |
|
|
|
6. Health protection agency (2010) Identification of E. coli
O157. National standard method BSOP ID 22 issue 3. http://www.hpa-standardmetods.org.uk/pdf_sops.asp,
Accessed on 1st Sept 2013. |
|
|
7. Riley, L.W., Remis R.S., Helgerson S.D., McGee, H.B.,
Wells, J.G., Gavis B.R., Hebert R.J., Olcott E.S., Johnson,
L.M., Hargrett, N.T., Blake, P.A. and Cohen, M.L. (1983)
Haemorrhagic colitis associated with rare Escherichia coli
Serotypes. New England Journal of Medicine, 308: 681-685.
http://dx.doi.org/10.1056/NEJM198303243081203
PMid:6338386 |
|
|
|
8. Hitchins, A.D., Hartman, P.A., Todd, E.C.D. (1992) Coliform
Escherichia coli and its toxins. In: Vanderzant, c.,
Splittstoesser, D. (Eds), compendium of methods for the
Microbiological Examination of foods. pp. 327-329. Compiled by
the APHA Technical Committee on Microbio- logical Methods for
Foods. |
|
|
|
9. Quinn, P.J., Carter, M.E., Markey, B.K. and Carter, G.R.
(1994) Clinical Veterinary Microbiology, pp. 21-26 and 209-
236. Published by Wolfe Publishing, an imprint of Mosby- Year
Book Europe Limited Printed in Spain by Grafos, S.A. Arte
SobrePapel ISBN 0723417113. |
|
|
10. Paton, A.W., and Paton,J.C. (1998) Detection and
characterization of Shiga toxigenic Escherichia coli by using
multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic
E. coli hlyA, rfbO111, and rfbO157. Journal of Clinical
Microbiology, 36:598-602.
PMid:9466788 PMCid:PMC104589 |
|
|
11. Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turck, M.
(1966) Antibiotic susceptibility testing by a standardized
single disc method. American Journal of Clinical Pathology,
45(4): 493-496.
PMid:5325707 |
|
|
|
12. Daood, N. (2007) Detection and antimicrobial
susceptibility ofE.coliO157: H7 in raw bovine milk, some of
dairy products and water samples. Damascus University Journal
for BasicSciences, 23:1-15. |
|
|
13. Rashid, M., Kotwal, S.K., Malik, M.A and Singh, M. (2013)
Prevalence, genetic profile of virulence determinants and
multidrug resistance of E. coli isolates from foods of animal
origin, Vet. World 6(3):139-142.
http://dx.doi.org/10.5455/vetworld.2013.139-142 |
|
|
14. Soomro, A.H., Arain, M.A., Khaskheli, M. and Bhutto, B.
(2002)Isolation of Escherichia Coli from Raw Milk and Milk
Products in Relationto Public Health Sold under Market
Conditions at Tandojam. Pakistan Journal of Nutrition, 1(3):
151-152.
http://dx.doi.org/10.3923/pjn.2002.151.152 |
|
|
|
15. Ali, A. A. and Abdelgadir, W.S. (2011) Incidence of
Escherichia coli in Raw Cow's Milk in Khartoum State. British
Journal of Dairy Sciences,2(1): 23-26. |
|
|
16. Adesiyun, A.A. (1994) Bacteriological quality and
associated public health risk of pre-processed bovine milk in
Trinidad. International Journal of Food Microbiology,
21:253-261.
http://dx.doi.org/10.1016/0168-1605(94)90032-9 |
|
|
|
17. Njage, P.M.K., Jans, C., Wangoh, J., Lacroix, C. and
Meile,L. (2012) Detection, isolation and molecular
characterisation of Shigatoxigenic O157 and non-O157
Escherichia coli in raw and fermented camel milk. African
Journal of Microbiology, 6(31):6031-6038. |
|
|
|
18. Steele, M.L., Mcnab, W.B., Poppe, C., Griffiths, M.W.,
Chen, S., Degrandis, S.A., Eruhner, L.C., Larkin, C.A., Lynch,
J.A. and Odumeru, J.A. (1997) Survey of Ontario bulk tank raw
milk for food-borne pathogens. Journal of Food Protection, 60:
1341-1346. |
|
|
19. Chye, F.Y., Abdullah, A., Ayob, M.K. (2004)
Bacteriological quality and safety of raw milk in Malaysia.
Food Microbiology, 21:535-541.
http://dx.doi.org/10.1016/j.fm.2003.11.007 |
|
|
|
20. Vicente, H.I.G., Amaral, L.A., Cerqueira, A.M.F. (2005)
ShigatoxigenicEscherichia coli serogroups O157, O111 and O113
in feces, water and milk samples from dairy farms. Brazil
journal Microbiology, 36: 217-222. |
|
|
21. Hussein, H.S., Sakuma, T. (2005) Prevalence of Shiga toxin
producing Escherichia coli in dairy cattle and their products.
Journal Dairy Science, 88: 450-465.
http://dx.doi.org/10.3168/jds.S0022-0302(05)72706-5 |
|
|
22. Rangel P. and Marin, J.M. (2009)Analysis of Escherichia
coli isolated from bovine mastitic milk. Pesquisa Veterinaria
Brasileira, 29(5): 363-368.
http://dx.doi.org/10.1590/S0100-736X2009000500001 |
|
|
|
23. Rahimi, E., Chaleshtori, S.S. and Parsaei, P.
2011.Prevalence and antimicrobial resistance of Escherichia
coli O157 isolated from traditional cheese, ice cream and
yoghurt in Iran. African Journal of Microbiology Research,
5(22): 3706-3710. |
|
|
24. Momtaz, H., Farzan, R., Rahimi, E., Dehkordi, F.S. and
Souod, N. (2012) Molecular Characterization of Shiga
Toxin-Producing Escherichia coli Isolated from Ruminant and
Donkey Raw Milk Samples and Traditional Dairy Products in
Iran. The Scientific World Journal, 10:1-13.
http://dx.doi.org/10.1100/2012/231342
PMid:22919299 PMCid:PMC3419413 |
|
|
