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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 27-10-2013)
18. Development of molecular tools to differentiate
Indian wild pig (Sus scrofa cristatus) meat from exotic and local domestic pig
meat - Kajal Kumar Jadav, Avadh Bihari Shrivastav and Nidhi Rajput
Veterinary World, 6(11): 919-922
doi:
10.14202/vetworld.2013.919-922
Abstract
Aim: Identification of wild
pig and domestic pig is essential to prevent illegal poaching of
wild pig and to implement Wildlife (Protection) Act, 1972.
PCR-RFLP was used to differentiate Wild pig (Sus scrofa cristatus)
from Domestic pig (Sus scrofa domestica) meat.
Materials and Methods: DNA was isolated from meat samples
of both the sub species and a fragment of Cytochrome b gene was
amplified using universal primers and the PCR products were
subjected to restriction digestion.
Results: All the known samples of each of the sub-species
amplified 474 bp fragment successfully using b1 and b2 primers. To
differentiate between wild and domestic pig meat, restriction
digestion of the PCR products was carried out to produce
characteristic PCR-RFLP patterns for each species. StuI digestion
yielded a RFLP pattern which distinguished the closely related sub
species. The alignment of sequences of Wild pigs with sequences of
local domestic pig, European wild pig and exotic breeds revealed 7
intra-species polymorphic sites within Cytochrome b gene fragment.
Conclusion: This study showed that The PCR-RFLP is a simple
and very effective tool for differentiating the samples of both
the sub species and could prove to be a useful tool in forensic
identification of wild pig and domestic pig.
Keywords: cytochrome b gene, domestic pig, Indian wild pig,
PCR-RFLP
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