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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 29-10-2013)
19. Detection and differentiation of sheeppox virus and
goatpox virus from clinical samples using 30 kDa RNA polymerase subunit (RPO30)
gene based PCR - R. Santhamani, R. Yogisharadhya, G. Venkatesan, S.
B. Shivachandra, A. B. Pandey and M. A. Ramakrishnan
Veterinary World, 6(11): 923-925
doi:
10.14202/vetworld.2013.923-925
Abstract
Aim: To detect and
differentiate Capripox virus (sheeppox virus and goatpox virus)
infections by using 30 kDa RNA polymerase subunit (RPO30) gene
based PCR.
Materials and Methods: Two capripox viruses' viz., sheep
pox virus (SPPV) and goatpox virus (GTPV) from clinical samples of
different outbreaks were detected and differentiated using capri
pox virus (CaPVs) genotyping PCR targeting the CaPV RPO30 gene. By
using the above PCR assay, a total of 54 scab samples from pox
disease outbreaks occurred in goats (n=21) and sheep (n=33) were
screened.
Results: Out of 54 clinical samples, 43 [17 out of 21
(80.95%) goat scabs and 26 out of 33 sheep (78.78%)] were found
positive for capripox virus infection. All positive samples
yielded expected amplicon sizes of 172 bp for goatpox virus and
152 bp for sheep pox virus.
Conclusion: The current study demonstrated that RPO30 gene
based PCR assay could be used for molecular epidemiology of
capripox virus infection and differentiation of causative agent
viz., sheep pox virus and goatpox virus.
Key words: capripox virus, differentiation PCR, goat pox,
RPO30 gene, sheeppox
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