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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 22-09-2013)
2. Inclusion of different exogenous fibrolytic enzymes
to dry jowar fodder and their effect on in vitro total gas production
- S.H. Sipai, K.S. Dutta, H.H. Savsani, K.S. Murthy, P.H. Vataliya, J.A. Chavda
and A.P. Gajera
Veterinary World, 6(11): 839-843
doi:
10.14202/vetworld.2013.839-843
Abstract
Aim: Our objective was to
estimate in-vitro gas production from dry jowar fodder added with
different concentrations of exogenous fibrolytic enzymes (EFEs)
like neutral cellulase and fungal xylanase.
Materials and Methods: 34 different samples of dry jowar
fodder were prepared according to different concentrations of
neutral cellulase, fungal xylanase and neutral cellulase + fungal
xylanase (1:1). Sample not containing any enzymes was considered
as the control group. These 34 samples were subjected to further
in vitro gas production analysis.
Results: Statistically, significantly higher (P<0.05)
potential gas production was recorded for 0.7 % at 6 hr period,
0.7 % at 12 hr period, 0.7 %, 0.8 % at 18 hr period and 0.7 %, 0.8
% at 24 hr period in the samples treated with neutral cellulase.
Significantly higher potential gas production was recorded for 0.5
%, 0.8 % at 6 hr period, 0.5 %, 0.6 %, 0.8 % at 12 hr period, 0.8
% at 18 hr period and 0.5 %, 0.6 %, 0.8 % at 24 hr period in the
samples treated with fungal xylanase. Significantly higher
potential gas production was recorded for 0.6 %, 0.6 %, 0.8 % at 6
hr period, 0.6 %, 0.8 % at 12 hr period, 0.6 %, 0.8 % at 18 hr
period and 0.6 %, 0.8 % at 24 hr period in the samples treated
with mixture of neutral cellulase + fungal xylanase (1:1).
Conclusion: Addition of neutral cellulase and fungal
xylanase into the samples of dry jowar fodder increased in vitro
total potential gas production. EFEs increase substrate
degradation and there by improve the nutritive value of dry jowar
fodder.
Keywords: dry jowar fodder, fungal xylanase, neutral
cellulase, total gas production
References
|
1. Beauchemin, K. A., Morgavi, D. P., McAllister, T. A., Yang,
W. Z. and Rode, L. M. (2001) The Use of Enzymes in Ruminant
Diets. In: Recent Advances in Animal Nutrition, Garnsworthy,
P.C. and J. Wiseman (Eds.). Nottingham University Press,
Loughborough, England, 297-322. |
|
|
|
2. Bandla, S., Chaturvedi, O. H., Malik. R., and Asgar, M.
(2010) Effect of enzyme to substrate ratio of exogenous
fibrolytic and protease enzymes on in vitro gas production
kinetics. The Indian Journal of Small Ruminants. 14(2):181-
190. |
|
|
|
3. Yancy M. I., Valli, C. and Balakrishnan, V. (2011)
Enhancing utilization of sorghum stover and groundnut haulm by
pre treating them with non – starch polysaccharidase mixture.
Tamilnadu Journal of Veterinary & Animal Sciences.
7(3):150-153. |
|
|
|
4. Vazquez, A. G., Martinez, G. M. and Rodriguez, J. P. (2011)
Comparison of in vitro degradation of elephant grass and
sugarcane by exogenous fibrolytic enzymes. African Journal of
Microbiology Research. 5(19): 3051-3053. |
|
|
|
5. Gallardo Ismael, Barcena Ricardo, Juan Manuel Pinos-
Rodriguez, Mario Cobos, Lorenzo Carreon and Maria Esther
Ortega (2010) Influence of exogenous fibrolytic enzymes on in
vitro and in sacco degradation of forages for ruminants.
Italian Journal of Animal Science. 9(1): 34-38. |
|
|
6. Bhat, M. K. (2000) Cellulases and related enzymes in
biotechnology. Biotechnol. Adv. 18: 355-383.
http://dx.doi.org/10.1016/S0734-9750(00)00041-0 |
|
|
7. Beg, Q. K., Kapoor, M., Mahajan, L. and Hoondal, G. S.
(2001) Microbial xylanases and their industrial applications:
a review. Appllied Microbiology and Biotechnology. 56:
326-338.
http://dx.doi.org/10.1007/s002530100704
PMid:11548999 |
|
|
8. Gado, H. M., Salem, A. Z., Robinson, P. H., and Hassan, M.
(2009) Influence of exogenous enzymes on nutrient
digestibility, extent of ruminal fermentation as well as milk
production and composition in dairy cows. Animal Feed Science
Technology. 154: 36-46.
http://dx.doi.org/10.1016/j.anifeedsci.2009.07.006 |
|
|
9. Alvarez, G., Pinos-Rodriguez, J. M., Herrera, J. G.,
Garcia, J. C., Gonzalez, S. S. and Barcena, R. (2009) Effects
of exogenous fibrolytic enzymes on ruminal digestibility in
steers fed high fibre rations. Livestock Science. 121: 150-
154.
http://dx.doi.org/10.1016/j.livsci.2008.05.024 |
|
|
10. Makkar, H. P. S., Blümmel, M. and Becker, K. (1995)
Formation of complexes between polyvinyl pyrrolidone and
polyethylene glycol with tannins and their implications in gas
production and true digestibility in in vitro techniques.
British Journal of Nutrition. 73: 897-913.
http://dx.doi.org/10.1079/BJN19950095
PMid:7632671 |
|
|
11. Blummel, M., Makkar, H. P. S. and Becker K. (1997) In
vitro gas production: a technique revisited. Journal of Animal
Physiology and Animal Nutrition. 77: 24-34.
http://dx.doi.org/10.1111/j.1439-0396.1997.tb00734.x |
|
|
12. Menke, K. H., Raab, L., Salewski, A., Steingass, H.,
Fritz, D. and Schneider, W. (1979) The estimation of the
digestibility and metabolizable energy content of ruminant
feedstuffs from the gas production when they are incubated
with rumen liquor in vitro. Journal of Agriculture Science. (Camb.).
92: 217-222.
http://dx.doi.org/10.1017/S0021859600086305 |
|
|
|
13. Steingass, H. (1983) Bestimmung des energetischen
Futterwertes von wirtschaftseigenen Futtermitteln aus der
Gasbildung bei der Pansenfermentation in vitro. PhD Thesis,
University of Hohenheim, Hohenheim, Germany. |
|
|
|
14. Snedecor, G. W. and Cochran, W. G. (1994) Statistical
Methods. 6th Edn., IOWA State University Press, USA.
pp258-298. |
|
|
15. Duncan, D. B. (1955) Multiple range and multiple F test.
Biometrics, 11: 1-42.
http://dx.doi.org/10.2307/3001478 |
|
|
|
16. Sallam, S. M. A. (2005) Nutritive value assessment of the
alternative feed resources by gas production and rumen
fermentation In vitro. Research Journal of Agriculture and
Biological Sciences, 1(2): 200-209. |
|
|
17. Vitti, D. M. S. S., Nozella, E. F., Abdalla, A. L., Buenoa,
I. C. S., Silva Filho, J. C., Costa, C., Buenod, M. S., Longo,
C., Vieira, M. E. Q., Cabral Filho, S. L. S., Godoy, P. B. and
Mueller-Harvey, I. (2005) The effect of drying and urea
treatment on nutritional and anti-nutritional components of
browses collected during wet and dry seasons. Animal Feed
Science Technology. 122: 123-133.
http://dx.doi.org/10.1016/j.anifeedsci.2005.04.007 |
|
|
|
18. Sallam, S. M. A., Bueno, I. C. S., Godoy, P. B., Nozella,
E. F., Vitti, D. M. S. S. and Abdalla, A. L. (2008) Nutritive
value assessment of the artichoke (Cynara scolymus) by-product
as an alternative feed resource for ruminants. Tropic
Subtropic Agroecosys. 8: 181- 189. |
|
|
|
19. Medjekal, S., Arhab, R. and Bousseboua, H. (2011)
Nutritive value assessment of some desert by-products by gas
production and rumen fermentation in vitro. Livestock Research
for Rural Development. 23 (3):217-222. |
|
|
20. Wallace, R. J., Wallace, S. J. A., McKain, N., Nsereko, V.
L., and Hartnell, G. F. (2001) Influence of supplementary
fibrolytic enzymes on the fermentation of corn and grass
silages by mixed ruminal microorganisms in vitro. Journal of
Animal Science. 79: 1905–1916.
PMid:11465379 |
|
|
21. Tang, S. X., Tayo, G. O., Tan, Z. L., Sun, Z. H., Shen, L.
X., Zhou, C. S., Xiao, W. J., Ren, G. P., Han, X. F. and Shen,
S. B. (2008) Effects of yeast culture and fibrolytic enzyme
supplementation on in vitro fermentation characteristics of
low-quality cereal straws. Journal of Animal Science. 86:
1164–1172.
http://dx.doi.org/10.2527/jas.2007-0438
PMid:18203979 |
|
|
22. Weimer, P. J. (1996) Why don't ruminal bacteria digest
cellulose faster? Journal of Dairy Science. 79: 1496-1502.
http://dx.doi.org/10.3168/jds.S0022-0302(96)76509-8 |
|
|
23. Jalilvand, G., Odongo, N. E., Lopez, S., Naserian, A.,
Valizadeh, F., Eftekhar Shahrodi, F., Kebreab, E. and France,
J. (2008) Effects of different levels of an enzyme mixture on
in vitro gas production parameters of contrasting forages.
Animal Feed Science Technoogyl. 146: 289-301.
http://dx.doi.org/10.1016/j.anifeedsci.2008.01.007 |
|
|
