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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 06-11-2013)
20. Seroepidemiology of canine leptospirosis by iELISA
and MAT -
R. Sumanth Kumar, R. M. Pillai, H. K. Mukhopadhyay, P. X. Antony,
J. Thanislass, V. M. Vivek Srinivas and S. Vishnupriya
Veterinary World, 6(11): 926-930
doi:
10.14202/vetworld.2013.926-930
Abstract
Aim: The present study was
conducted to determine the prevalence of antibodies against
leptospira using a genus specific and serovar specific antigen by
indirect ELISA (iELISA) and Microscopic Agglutination test (MAT),
respectively.
Materials and Methods: 300 sera samples were collected from
dogs, from Pondicherry, suspected to be suffering from Canine
leptospirosis and with no history of vaccination. Using the outer
membrane proteins (OMP's) of Leptospira interrogans serovar
canicola as antigen the leptospiral antibodies were detected by
iELISA and compared with the MAT using five commonly infecting
serovars (canicola, icterohaemorrhaegiae, grippotyphosa, pomona,
australis) as antigens. Statistical analysis of the data was
carried out using chi-square test.
Result: Out of 300 sera samples, 159 (53 %) and 99 (33 %)
sera samples were found to be positive for leptospiral antibodies
by iELISA and MAT, respectively. The iELISA employed in this study
revealed a very high sensitivity of 94.94 % in comparison with the
MAT (59.11 %). However, iELISA showed less specificity (67.66 %)
when compared to MAT (96.45 %). By chi-square test, a significant
difference (P<0.001) was obtained between the results from iELISA
and MAT.
Conclusion: The present study revealed that the iELISA was
found to be highly sensitive, rapid and easy to perform in
comparison with MAT for the detection of canine anti-leptospiral
antibodies.
Key words: Canine leptospirosis, outer membrane protein (OMP),
iELISA, Microscopic Agglutination test (MAT).
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