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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 28-09-2013)
4. Evaluation and development of therapeutic management
of subacute toxicity of Jatropha curcas seed oil in goats - Amit
Shukla and Satyapal Singh
Veterinary World, 6(11): 852-856
doi:
10.14202/vetworld.2013.852-856
Abstract
Aim: The present study was
designed to evaluate and develop the therapeutic management for
toxicity of Jatropha curcas seed oil in goats.
Materials and Methods: Sub-acute toxicity of J. curcas seed
oil was evaluated by using nine healthy goats, weighing 28-32 kg
and 16-18 months old, equally and randomly divided in three groups
(n = 3 per group). Adequate measures were taken to minimize pain
to the animals. Group I served as control; they did not receive
any treatment. The study was conducted by daily oral dosing of J.
curcas seed oil @ 1ml/ kg body weight followed by the treatment
with the therapeutic module comprising of sodium thiosulphate (50mg/ kg bodyweight) and glutathione (0.25 mg/ kg body weight)
in group II; whereas, goats in group III were given daily oral
dosing of J. curcas seed oil only for 28 days. Hematobiochemical
and antioxidative parameters as Lipid Peroxidase (LPO) and Reduced
Glutathione (GSH) in erythrocytes were estimated at day 0, 14 and
28 days.
Results: Mild to moderate diarrhoea, dullness, depression
and letharginess were observed in all animals in group III,
whereas, mild clinical signs were observed in treatment group II.
A significant (P<0.05) decrease in Hb , PCV, TEC ,TLC , total
serum protein , albumin and globulin values were observed in group
II and III in comparison to the control group I, however, the
group II showed increase in the aforesaid parameters in comparison
with without group III indicating the therapeutic efficacy of the
therapeutic module. A significant (P<0.05) increase in serum
creatinine, serum urea, cholesterol, AST, ALT and ALP were
observed in all the groups. Antioxidative parameters in
erythrocytes as LPO and GSH increased in group III in comparison
to the control and group II.
Conclusion: It is concluded from the above study that
therapeutic module comprising of sodium thiosulphate (50mg/kg IV)
and glutathione (0.25 mg/kg IM) in sub-acute study reduces the
toxic effects of J. curcas seed oil as indicated by the improved
Hematobiochemical values in the with treatment group II than the
without treatment group III.
Keywords: curcin, Jatropha curcas, phorbol esters, toxicity
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