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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 28-09-2013)
5. Molecular characterisation of membrane glycoprotein
and 5b protein of nephropathogenic infectious bronchitis virus - A.
Balasubramaniam, K. Sukumar, P. Suresh and B. Puvarajan
Veterinary World, 6(11): 857-861
doi:
10.14202/vetworld.2013.857-861
Abstract
Background: Infectious
bronchitis virus (IBV) affects the upper respiratory tract and the
reproductive tract, and some strains can cause nephritis. Large
number of serotypes and genotypes of the virus have been
identified and for the most part do not cross-protect. Identifying
the genotype or serotype of IBV field strains is empirical for
selecting an appropriate candidate to serve as vaccine strain for
prevention of infectious bronchitis (IB) disease in chickens. The
variant strains of IBV could be circulating among chickens in
India and recently nephropathogenic IB was reported. Hence, the
present work was aimed to carry out molecular characterization of
nephropathogenic IBV isolates obtained from two different
geographical locations of south India, involving relatively
conserved regions.
Materials and Methods: MIBVPCR and NIBVPCR primers were
used to amplify partial M and N gene after synthesizing cDNA.
Isolates Ind/KA/07/1 and Ind/TN/07/2 were subjected to direct
sequencing as these two isolates are two different geographical
regions and scored better in induction of lesions during
pathotyping.
Results: Amino acids of membrane glycoprotein varied on
five occasions for the isolate Ind/KA/07/1 when aligned with that
of M 41 strain and isolate Ind/TN/07/2. Eight amino acids of both
isolates in 5b protein were different from that of M41 strain. Few
point mutations, short deletions and insertions were noticed in
the amplified genome, based on the membrane protein nucleotide
sequence comparison.
Conclusion: Prevalence of IBV strains with few
modifications in conserved regions indicated that there was
presence of variant IBV in south India.
Keywords: 5b protein, infectious bronchitis virus, membrane
protein, molecular characterization
References
1. Jackwood, M.W. (2012) Review of infectious bronchitis virus
around the world. Avian Dis., 56:634–641.
http://dx.doi.org/10.1637/10227-043012-Review.1
PMid:23397833 |
|
|
|
2. Cavanagh, D., and J. Gelb Jr. Infectious bronchitis. (2008)
In: Diseases of poultry, 12th ed. Saif, Y.M., Fadly, A.M.,
Glisson, J.R., McDougald, L.R., Nolan, L.K. and Swayne, D.E.
eds. Blackwell Publishing, Ames, IA. pp. 117–135. |
|
|
3. Elankumaran, S., C. Balachandran, N. D. Chandran, P. Roy,
A. Albert, and R. Manickam. (1999) Serological evidence for a
793B related avian infectious bronchitis virus in India. Vet.
Rec. 144:299–300.
PMid:10204229 |
|
|
4. Bayry, J., Goudar, M.S., Nighot, P.K., Kshirsagar, S.G.,
Ladman, B.S., Gelb Jr., J., Ghalsasi, G.R. and Kolte, G.N.
(2005) Emergence of a nephropathogenic avian infectious
bronchitis virus with a novel genotype in India. J. Clin.
Microbiol., 43: 916 - 918.
http://dx.doi.org/10.1128/JCM.43.2.916-918.2005
PMid:15695705 PMCid:PMC548090 |
|
|
|
5. Singh, F., Shakya, S. and Rawat, N. (2009) An outbreak of
nephropathogenic avian infectious bronchitis in broiler flocks
of Chhattisgarh region. Indian Journal of Poultry Science
44:379-381. |
|
|
6. Bing G.X., Liu, X, Pu, J., Liu, Q.F., Wu, Q.M. and Liu, J.H.
(2007) Different genotypes of nephropathogenic infectious
bronchitis virus co-circulating in chicken population in
China. Virus Genes 35: 333-337.
http://dx.doi.org/10.1007/s11262-007-0100-5
PMid:17431755 |
|
|
7. Han, Z., Sun, C. Yan, B., Zhang, X., Wang, Y., Li, C.,
Zhang, Q., Ma, Y., Shao, Y. Liu, Q., Kong, X. and Liu, S.
(2011) A 15-year analysis of molecular epidemiology of avian
infectious bronchitis coronavirus in China. Infect. Genet.
Evol., 11:190–200.
http://dx.doi.org/10.1016/j.meegid.2010.09.002
PMid:20833270 |
|
|
8. Ji, J., Xie, J., Chen, F., Shu, D., Zuo, K., Xue, C., Qin,
J., Li, H., Bi, Y., Ma, J. and Xie, Q. (2011) Phylogenetic
distribution and predominant genotype of the avian infectious
bronchitis virus in China during 2008–2009. Virol. J., 8:184.
http://dx.doi.org/10.1186/1743-422X-8-184
PMid:21510909 PMCid:PMC3094301 |
|
|
9. Krapez, U., Slavec, B. and Rojs, O.Z. (2011) Circulation of
infectious bronchitis virus strains from Italy 02 and QX
genotypes in Slovenia between 2007 and 2009. Avian Dis.,
55:155–161.
http://dx.doi.org/10.1637/9533-091710-Case.1
PMid:21500655 |
|
|
10. Ariyoshi, R., Kawai, T., Honda, T. and Tokiyoshi, S.
(2010) Classification of IBV S1 genotypes by direct reverse
transcriptase–polymerase chain reaction (RT-PCR) and
relationship between serotypes and genotypes of strains
isolated between 1998 and 2008 in Japan. J. Vet. Med. Sci.,
72:687–692.
http://dx.doi.org/10.1292/jvms.09-0130
PMid:20093802 |
|
|
11. Mase, M., Kawanishi, N., Ootani, Y., Murayama, K., Karino,
A., Inoue, T. and Kawakami, J. (2010) A novel genotype of
avian infectious bronchitis virus isolated in Japan in 2009.
J. Vet. Med. Sci., 72:1265–1268.
http://dx.doi.org/10.1292/jvms.10-0080
PMid:20460833 |
|
|
12. Acevedo, A.M., Diaz de Arce, H., Brandao, P.E., Colas, M.,
Oliveira, S. and Perez, L.J. (2012) First evidence of the
emergence of novel putative infectious bronchitis virus
genotypes in Cuba. Res. Vet. Sci., 93:1046–1049.
http://dx.doi.org/10.1016/j.rvsc.2012.01.012
PMid:22357366 |
|
|
13. Lim, T.H., Lee, H.J., Lee, D.H., Lee, Y.N., Park, J.K.,
Youn, H.N., Kim, M.S., Lee, J.B. Park, S.Y., Choi, S. and
Song, C.S. (2011) An emerging recombinant cluster of
nephropathogenic strains of avian infectious bronchitis virus
in Korea. Infect. Genet. Evol., 11:678–685.
http://dx.doi.org/10.1016/j.meegid.2011.01.007
PMid:21255688 |
|
|
14. Ovchinnikova, E.V., Bochkov, Y.A., Shcherbakova, L.O.,
Nikonova, Z.B., Zinyakov, N.G., Elatkin, N.P., Mudrak, N.S.,
Borisov, A.V. and Drygin, V.V. (2011) Molecular
characterization of infectious bronchitis virus isolates from
Russia and neighbouring countries: identification of
intertypic recombination in the S1 gene. Avian Pathol.,
40:507– 514.
http://dx.doi.org/10.1080/03079457.2011.605782
PMid:21854179 |
|
|
15. Fraga, A.P., Balestrin, E., Ikuta, N., Fonseca A.S.K.,
Spilki, F.R., Canal, C.W. and Lunge, V.R. (2013) Emergence of
a new genotype of avian infectious bronchitis virus in Brazil.
Avian Dis., 57:225-232.
http://dx.doi.org/10.1637/10346-090412-Reg.1 |
|
|
16. Kang, S.C., Eun, K.L., Woo, J.J., Mi, J.P. Jin, W.K. and
Jun, H.K. (2009) Pathogenicity and antigenicity of a new
variant of Korean nephropathogenic infectious bronchitis
virus. J. Vet. Sci., 10: 357-359.
http://dx.doi.org/10.4142/jvs.2009.10.4.357
PMCid:PMC2807275 |
|
|
|
17. Pohuang, T. and Sasipreeyajan, J. (2012) The pathogenesis
of a new variant genotype and QX like infectious bronchitis
virus isolated from chickens in Thailand. Thai J. Vet. Med.,
42: 51-57. |
|
|
|
18. Ababneh, M., Dalab, A.E., Alsaad, S. and Al-zghoul, M.
(2012) Presence of infectious bronchitis virus strain CK/ CH/
LDL/97I in the Middle East. Vet. Sci., doi:10.5402/2012/2
01721. |
|
|
19. Mahmood, Z.H., Sleman, R.R. and Uthman, A.U. (2011)
Isolation and molecular characterization of Sul/01/09 avian
infectious bronchitis virus, indicates the emergence of a new
genotype in the Middle East. Vet. Microbiol., 150:21–27.
http://dx.doi.org/10.1016/j.vetmic.2010.12.015
PMid:21216111 |
|
|
20. Andreasen, J. R., Jackwood, M. R. and Hilt, D.A. (1991)
Polymerase chain reaction amplification of the genome of
infectious bronchitis virus. Avian Dis., 35: 216-220.
http://dx.doi.org/10.2307/1591317
PMid:1851417 |
|
|
21. Wang, C.H. and Huang Y.C. (2000) Relationship between
serotypes and genotypes based on the hypervariable region of
the SI gene of infectious bronchitis virus. Arch. Virol., 145:
291- 300.
http://dx.doi.org/10.1007/s007050050024
PMid:10752554 |
|
|
|
22. Jones, R.C., Worthington, K.J., Capua, I.and Naylor, C.J.
(2005) Efficacy of live infectious bronchitis vaccines against
novel European genotype, Italy 02. Vet. Rec., 156: 6 -647. |
|
|
23. de Wit, J. J., J. K. Cook, and H. M. van der Heijden.
(2011) Infectious bronchitis virus variants: a review of the
history, current situation and control measures. Avian Pathol.,
40: 223–235.
http://dx.doi.org/10.1080/03079457.2011.566260
PMid:21711181 |
|
|
24. Yu, L., Jiang, Y. Low, S. Wang, Z. Nam, S.J. Liu W.L. and
Kwang, J. (2001) Characterization of three infectious
bronchitis virus isolates from China associated with
proventriculus in vaccinated chickens. Avian Dis., 45: 416 -
424.
http://dx.doi.org/10.2307/1592981
PMid:11417821 |
|
|
25. Farsang, A., Ros, C, Renstrom, H. M. Baule, C. Soos, Y.
and Selak, S. (2002) Molecular epizootiology of infectious
bronchitis virus in Sweden indicating the involvement of
vaccine strain. Avian Pathol., 31: 229 - 236.
http://dx.doi.org/10.1080/03079450220136530
PMid:12396345 |
|
|
26. Abro, S.H., Renstrom, L.H., Ullman, K., Isaksson, M.,
Zohari, S., Jansson, D.S., Belak, S. and Baule, C. (2011)
Emergence of novel strains of avian infectious bronchitis
virus in Sweden. Vet. Microbiol., 155:237–246.
http://dx.doi.org/10.1016/j.vetmic.2011.09.022
PMid:22005179 |
|
|
27. Zulperi, Z.M., Omar, A. R. and Arshad, S. S. (2009)
Sequence and Phylogenetic analysis of S1, S2, M, and N genes
of infectious bronchitis virus isolates from Malaysia. Virus
Genes, 38: 383-391.
http://dx.doi.org/10.1007/s11262-009-0337-2
PMid:19242786 |
|
|
28. Zwaagstra, K.A., Van Zeijst, B.A.M. and Kusters, J.G.
(1992) Rapid detection and identification of avian infectious
bronchitis virus. J. Clini. Microbiol., 30: 79 - 84.
PMid:1310335 PMCid:PMC265000 |
|
|
29. Li, H. and Yang, H. (2001) Sequence analysis of
nephropathogenic infectious bronchitis virus strains of the
Massachusetts genotype in Beijing. Avian Pathol., 30: 535
-541.
http://dx.doi.org/10.1080/03079450120078734
PMid:19184943 |
|
|
30. Mardani, K., Noormohammadi, A.H., Ignjatovic, J. and
Browning, G.F. (2010) Naturally occurring recombination
between distant strains of infectious bronchitis virus. Arch.
Virol., 155:1581–1586.
http://dx.doi.org/10.1007/s00705-010-0731-z
PMid:20574643 |
|
|
