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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 05-10-2013)
7. Protective role of turmeric on histological,
ultrastructural and sero-biochemical changes in cisplatin-induced nephrotoxicity
in female rats - B. Ramya, Y. Anjaneyulu, A. Gopala Reddy, D. Madhuri,
M. Lakshman and P. Shivakumar
Veterinary World, 6(11): 865-869
doi:
10.14202/vetworld.2013.865-869
Abstract
Aim: Protective role of
turmeric was assessed against cisplatin-induced histological and
ultra-structural changes in kidney.
Materials and Methods: A total of 48 rats were divided into
4 groups of 12 rats in each. Group 1 was kept as sham control,
group 2 was treated with cisplatin (@ 2 mg/kg b.wt,
intraperitoneally on day 1, 7, 14 and 21), group 3 with turmeric
(@ 0.05 mg/kg b.wt. p.o. once daily for 28 days) and group 4 with
cisplatin + turmeric (as per above schedule). Blood was collected
at fortnight intervals and serum was separated for estimation of
kidney biomarkers. Six rats in each group were then euthanized on
day 14 and 28 for histopathology, and tissue parameters were
assayed on day 28.
Results: Thibarbituric acid reacting substances (TBARS),
protein carbonyls, serum creatinine, blood urea nitrogen (BUN)
were significantly (P < 0.05) increased, while GSH was
significantly (P < 0.05) decreased in group 2 as compared to other
groups. Histological sections of kidney from group 2 rats showed
marked inter-tubular haemorrhages, congestion, marked dilation of
tubules, and other lesions of pathological significance.
Ultrastructural changes of pathological significance were also
recorded in group 2. Kidney sections of group 4 showed nucleus
with uniform size with well differentiated nuclear membrane and
nucleolus, and prominent inter tubular and inter cellular
junctions.
Conclusion: The study revealed that cisplatin induces
nephrotoxicity due to oxidative stress and turmeric was found
beneficial in countering the adverse effects.
Key words: cisplatin, nephrototoxicity, oxidative stress,
turmeric.
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