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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 13-08-2013)
10.
Molecular epidemiology of canine parvovirus in southern
India - V. M. Vivek Srinivas, H. K. Mukhopadhyay, J. Thanislass, P. X. Antony and R. M.
Pillai
Veterinary World, 6(10): 744-749
doi:
10.14202/vetworld.2013.744-749
Abstract
Aim:
The present study was conducted to isolate and characterize
canine parvovirus circulating in Southern India by genetic
analysis of VP2 capsid protein gene.
Materials and Methods: In this study, 128 samples were
collected from nine different locations covering five Southern
Indian states (Pondicherry, Tamil Nadu, Kerala, Andhra Pradesh and
Karnataka) . Out of 128 samples, 69 samples were found to be
positive by PCR assay. Out of 69 positive samples, 36 were
randomly selected and processed for virus isolation. Twenty
viruses could be isolated successfully and 18 randomly selected
isolate were subjected to VP2 gene sequence analysis along with 6
random clinical samples.
Result:
Seventeen isolates and 5 clinical samples were characterized
as New CPV-2a (CPV2a with 297-Ser →Ala).
But one isolate and one clinical sample had amino acids variations
which were characteristics of New CPV-2b. The phylogenetic
analysis revealed that one of the field isolates was found to be
phylogenetically closely related to New CPV-2b strains of India;
rest other sequences was found to share ancestral origins with New
CPV-2a reference strains of Japan, China, Thailand and India.
Conclusion: The present study revealed that the predominant
CPV strain circulating in Southern India is New CPV-2a. There is
also enough indication of New CPV-2b strain from different states
of Southern India.
Keywords: Canine parvovirus (CPV), crandell feline kidney (CRFK)
cell line, molecular epidemiology, phylogenetic analysis, sequence
analysis.
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