|  | 
              
  
              
              Open Access  
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, 
distribution and reproduction in any medium, provided the work is properly 
cited. 
 
              
              
              Research (Published 
online :  19-08-2013) 
16. Various causes related to dead-in-shell embryos of crossbred (PB-2 x 
Indigenous) chicken egg - N. Kalita, N. Pathak, M. Ahmed and G. K. 
SaikiaVeterinary World, 6(10): 774-777
 
                
              doi: 
              10.14202/vetworld.2013.774-777 
                
              
              
          
 
              Abstract 
 
              Aim: The present study was 
              undertaken to study the etiopathology of dead-in-shell embryos of 
              PB-2 male x Indigenous female crossbred chicken egg.Materials and Methods: A total of 1377 eggs were incubated 
              which was collected from a flock of crossbreed bird 
              (PB-2xIndigenous) chicken. Out of which 568 (41.25 %) egg failed 
              to pip out, were utilized for further study. All the dead in shell 
              embryos were examined for different anomalies and pathological 
              condition thorough necropsy examination. For bacteriological 
              isolation a piece of liver, lung and yolk sac contents were 
              collected from 25 nos. of dead in shell embryos and send to the 
              Department of Microbiology for further examination.
 Results: A total of 241 (42.42 %) egg were recorded as 
              dead-in-shell embryos out of 568 eggs which were fail to pip out. 
              The percentage of dead-in-shell was higher on 21st day (61.34%) 
              than 18th day (38.66 %) of incubation. Out of 241 nos. of dead in 
              shell embryos, 47 (19.50%) cases showed malpositions,19 (7.88%) 
              malformation, 6 (2.49%) adhesion,4 (1.66%) dehydra-tion, 67 
              (27.80%) pathological condition and 98 (40.66%) cases showed no 
              definite abnormalities and 327 (57.57%) numbers of egg were found 
              as infertile.
 Conclusion: The dead in shell embryo may be due to genetic 
              factor, breed, some pathological condition, frequent power 
              failure, lack of proper hygiene etc.
 Keywords: crossbred chicken egg, dead in shell embryo, 
              etiopathology.
 
 
              References 
 
                
                  | 1. Haleem, M.A. (2009) Panel discussion on meat and poultry 
                  global trends challenges and their redresses. Poult. Planner 
                  (Supplementary Issue) - ILDEX India 2009.11(4):8. |  
                  |  |  
                  | 2. Goswami, J. (2009) Etiopathology of mortality in broiler 
                  chicks. M.V.Sc. Thesis submitted to Assam Agricultural 
                  University, Khanapara, Guwahati, India. |  
                  |  |  
                  | 3. Schaal, T. and Cherian, G.(2007) A survey of the 
                  hatchability of broiler and turkey eggs in the United States 
                  from 1985 through 2005. Poult. Sci.86:598-600. PMid:17297174
 |  
                  |  |  
                  | 4. Sankhyan, V., Katoch, S., Thakur, Y.P., Dogra, P.K. and 
                  Bhardwaj, N. (2012) Non- genetic factors affecting fertility, 
                  hatchability and chick survivability in sub temperate Palampur 
                  region of Himachal Pradesh. Proceed. of XXIX Anl. Conf. and 
                  Natnl. Symp. of Ind. Poult. Sci. Assoc. 5-7 December, 
                  Rajendranagar, Hyderabad, Ind., pp.17 (Abstr.). |  
                  |  |  
                  | 5. Henry, A.C.E., Jagatheesan, P.N.R., Paramasivam, A. and 
                  Mehala, C. (2012) Fertility and hatchability of Nandanam-I, 
                  Nandanam-II and desi varities of turkey. Proceed. of XXIX Anl. 
                  Conf. and Natnl. Symp. of Ind. Poult. Sci. Assoc. 5-7 
                  December, Rajendranagar, Hyderabad, Ind., pp.9 (Abstr.). |  
                  |  |  
                  | 6. Rajkumar, U., Niranjan, M., Rajarabindra, K.S., Padhi, M.K., 
                  Bhattacharya, T.K. and Chatterjee, R.N. (2012) Evaluation of 
                  juvenile and production performance in Grampriya male line 
                  chicken. Proceed. of XXIX Anl. Conf. and Natnl. Symp. of Ind. 
                  Poult. Sci. Assoc. 5-7 December, Rajendranagar, Hyderabad, 
                  India, pp.7 (Abstr.). |  
                  |  |  
                  | 7. Tona, K., Bamelis, F., Coucke, W., Bruggeman, V. and 
                  Decuypere, E. (2001) Relationship between broiler breeders's 
                  age and egg weight loss and embryonic mortality during 
                  incubation in large scale conditions. J. App. Poult. Res. 
                  10:221-227. |  
                  |  |  
                  | 8. Singh, D., Reddy, B.L.N. and Bhanja, S.K. (2012) Studies on 
                  incubation parameters in coloured broiler (PB-I) population 
                  over generations. Proceed. of XXIX Anl. Conf. and Natnl. Symp. 
                  of Ind. Poult. Sci. Assoc. 5-7 December, Rajendranagar, 
                  Hyderabad, Ind., pp.225 (Abstr.). |  
                  |  |  
                  | 9. Sanctuary, W.C. (1925) One cause of dead in chick in shell. 
                  Poult. Sci. 4:141-153. http://dx.doi.org/10.3382/ps.0040141
 |  
                  |  |  
                  | 10. Amer, M.F. (1962) Embryonic mortality and malpositions in 
                  Fayoumi chicken. Poult. Sci. 41:1702-1712. http://dx.doi.org/10.3382/ps.0411707
 |  
                  |  |  
                  | 11. Rudraprasad, V.C., Vijayasarathi, S.K. and Sreenivas, 
                  G.R.N.(1996) Malpositions in dead-in-shell chicken embryos. 
                  Indian J. Poult. Sci. 31 (1):56-59. |  
                  |  |  
                  | 12. Wilson, H.R., Neuman, S.L., Eldred, A.R. and Mather, F.B. 
                  (2003) Embryonic Malpositions in Broiler Chickens and Bobwhite 
                  Quail. J. App. Poult. Res. 12:14-23. |  
                  |  |  
                  | 13. Sharaf, M.M., Taha, A.E. and Ahmed, N.A.E. (2010) 
                  Deformities and abnormalities of Egyptian chicken embryos as 
                  reason of poor hatchability percentages. Egypt. Poult. Sci.30 
                  (1):317-337. |  
                  |  |  
                  | 14. Kalita, N., Barua, N., Pathak, N. and Islam, R. (2011) 
                  Etiopathology of dead in shell embryos of indigenous chicken 
                  egg. Indian J. Poult. Sci. 46(2):275-277. |  
                  |  |  
                  | 15. Kalita, N., Pathak, N., Ahmed, M. and Saikia, G.K. (2012) 
                  Various cause related to dead in shell embryos of Vanaraja and 
                  Crossbred chicken egg. Proceed. of XXIX Anl. Conf. and Natnl. 
                  Symp. of Ind. Poult. Sci. Assoc. 5-7 December, Rajendranagar, 
                  Hyderabad, Ind., pp.133 (Abstr.). |  
                  |  |  
                  | 16. Das, B.C. (1973) An investigation in to the mortality of 
                  embryos and brooder house chicks. M.V.Sc. Thesis. Orissa 
                  University of Agriculture and Technology, India. |  
                  |  |  
                  | 17. Vyas, R. and Bhatnagar, D.S. (1966) Embryonic mortality 
                  and malpositions in white leghorn breeds of poultry. Indian 
                  Vet. J.43:524-533. PMid:5949501
 |  
                  |  |  
                  | 18. Landauer, W. (1961) The hatchability of chicken eggs as 
                  influenced by environment and heredity. Connecticut Agric. 
                  Exp. Sta. Monograph 1.(c.f.Das,1973). |  
                  |  |  
                  | 19. Tona, K., Bruggeman, V., Onabgesan, O., Bamelis, F., 
                  Gbeassor, M., Mertens and Decuypere, E.(2005) Day old chick 
                  quality: Relationship to hatching egg quality, adequate 
                  incubation practice and prediction of broiler performance. 
                  Avian. and Poult. Biol. Res.16:109-119. http://dx.doi.org/10.3184/147020605783438787
 |  
                  |  |  
                  | 20. Rodriguez, B. and Juarez, L.(2008) Developmental delay and 
                  other anomalies in the offspring from hens immunized against 
                  soluble and foreign chick embryo antigen. American J. Reprd. 
                  Immunol. 60 (2):141-150. http://dx.doi.org/10.1111/j.1600-0897.2008.00606.x
 PMid:18705841
 |  
                  |  |  
                  | 21. Wannop, C. (1968) Some abnormalities of the chick embryo. 
                  Labortory Animal 2:191-194. http://dx.doi.org/10.1258/002367768781082843
 |  
                  |  |  
                  | 22. Borzemska, W., Piusinski, W., Malec, H. and Niedzioka, J. 
                  (1990) Pathomorphological changes in dead chick embryos from 
                  incubation at low values of cooling. Medicyna. Weterynaryjna. 
                  46: 103-105. |  
                  |  |  
                  | 23. Al-Sadi, H.I., Basher, H.A. and Ismail, H.K. (2000) 
                  Bacteriologic and pathologic studies on dead-in-shell embryos. 
                  Iraqi J. Vet. Sci. 13(2):297-307. |  
                  |  |  
                  | 24. Raji, M., Adekeye, J., Kwaga, J., Bale, J. and Henton, M. 
                  (2007) Serovars and biochemical characterization of 
                  Escherichia coli isolated from colibacillosis cases and dead- 
                  in-shell embryos in poultry in Zaria, Nigeria. Vet. Arhiv 
                  77(6):495-505. |  
                  |  |  
                  | 25. Muhammad, M., Muhammad, L.U., Mani, A.U. and Ambali, 
                  A.G.(2009) A survey of chick mortality at hatching in three 
                  selected hatcheries in Jos, central Nigeria. Int. J. Poult. 
                  Sci.8 (7):656-65 9. |  
                  |  |  
                  | 26. Jahantigh, M. (2010) Bacteriological study of 
                  Dead-in-Shell embryos of ostrich. Indian J. Vet. 
                  Res.11(1):88-90. |  
                  |  |  
                  | 27. Mazengia, H., Alemu, S., Mekuriaw, G. and Wuletaw, Z. 
                  (2012) Embryo mortality and isolation of E. coli as cause of 
                  death for in shell chick embryos and first week chicks. 
                  American. J. Vet.Med.Res.1(2): 33-36. |  
                  |  |  
                  | 28. Phangcho, C.V. and Mukit, A. (2002) Isolation of bacterial 
                  and fungal agents from dead in shell embryos of domestic 
                  pigeon (Columba livia). Indian J. Poult. Sci. 37:209-210. |  
                  |  |  
                  | 29. Shane, S.(1999) Promoting chick quality and liveability. 
                  ASA. Tech. Bull. 43:1-3. |  
                  |  |  
                  | 30. Das, R.K., Mishra, U.K. and Mishra, P.R. (1994) Embryonic 
                  death of chicks: a suevey. Indian Vet. J. 71:975-977. |  |  |