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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 11-09-2013)
25. Genetic characterization of Coxiella burnetii in Amblyomma varigatum ticks
from North-central Nigeria: public health importance - Ndudim Isaac
Ogo, Isabel Garcia Fernandez de Mera, Ruth Cecilia Galindo,
Oluyinka O Okubanjo, Hajiya Mairo Inuwa, Rowland I. S. Agbede and Jose de La
Fuente
Veterinary World, 6(10): 818-822
doi:
10.14202/vetworld.2013.818-822
Abstract
Aim: The purpose of this
pilot study was to genetically identify and characterize Coxiella
burnetii from Amblyomma varigatum ticks collected on cattle in
North central Nigeria.
Materials and Methods: A total of 40 partially fed ticks
morphologically identified as adult A. variegatum ticks collected
from cattle owned by Fulani pastoralists were evaluated for the
presence of C. burnetii using PCR, cloning, and sequencing of the
heat shock polypeptide gene htpB.
Results: C. burnetii DNA was detected in 10 (25%) of the
ticks analyzed. Sequences for the C. burnetii gene htpB detected
in our samples had 99-100% identity to all other C. burnetii that
have been described and that are deposited in the GenBank
database. Phylogenetic analysis using neighbor-joining method
indicates the clustering of C. burnetii sequences from our study
areas with those collected from Oyo state, South-western Nigeria
and Spain.
Conclusion: This study shows a high infection rate of C.
burnetii in A. variegatum ticks in the study areas. Phylogenetic
inferences indicates that the strain of C. burnetii found in the
North central states of Plateau and Nasarawa were same as those
previously reported in the South western state of Oyo. The
presence of this pathogen in naturally occurring A. variegatum
tick populations could present an additional risk of Q-fever
disease to humans, especially to the pastoralists that are closely
associated with their animals and are easily exposed to tick
bites. Therefore, further studies are needed to assess the
competence of A. variegatum ticks as vectors of C. burnetii
pathogens.
Keywords: Amblyomma variegatum, Coxiella burnetii, Nigeria,
ticks, zoonosis
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