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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 08-08-2013)
7. In vitro blastocyst development of post-thaw
vitrified bovine oocytes - D. J. Dutta, Hiramoni Dev and Himangshu
Raj
Veterinary World, 6(10): 730-733
doi:
10.14202/vetworld.2013.730-733
Abstract
Aim: To evaluate the
developmental competence of post-thaw vitrified bovine cumulus-oocyte
complexes (COCs) in vitro.
Materials and Methods: A total of 129 COCs were
cryopreserved using vitrification solution comprising of 15%
ethylene glycol (EG) + 15% dimethyl sulfoxide (DMSO) + 0.6 M
sucrose in medium TCM-199 with 10% FBS. Immediately, within a
minute they are plunged into liquid nitrogen using 0.25 ml straws.
Thawing was made with a step wise dilution method. Post-thaw
normal vitrified and non-vitrified oocytes were subjected to in
vitro maturation and in vitro fertilization.
Results: Post-thaw survival percentage of vitrified oocytes
was 88.37% and maturation performance of vitrified oocytes on the
basis of cumulus expansion was 81.58% as compared to non-vitrified
control 93.85%. The in vitro fertilization performance of
vitrified oocytes was 49.46% as compared to the non-vitrified ones
(63.11%). Similarly, blastocyst formation of vitrified oocytes was
21.74% as compared to 32.47% in non-vitrified oocytes.
Conclusion: Vitrification of immature bovine oocytes using
7.5% EG + 7.5% DMSO for equilibration and 15% EG +15% DMSO + 0.6 M
sucrose as vitrification solution yielded better in vitro
fertilization and blastocyst formation rate.
Keywords: cumulus-oocyte complexes, in vitro fertilization,
in vitro maturation, oocytes, vitrification
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