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              Open Access  
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, 
distribution and reproduction in any medium, provided the work is properly 
cited. 
 
              
              
              Research (Published 
online :  11-08-2013) 
9. 
Isolation and antibiogram of 
Staphylococcus, 
Streptococcus and Escherichia coli isolates from clinical and 
subclinical cases of bovine mastitis - 
Nihar Nalini Mohanty, Priyaranjan Das, Shaswati 
Subhadarsini Pany, Laxmi Narayan Sarangi, Siddharth Ranabijuli and Hemant Kumar 
PandaVeterinary World, 6(10): 739-743
 
                
              doi: 
              10.14202/vetworld.2013.739-743 
                
              
              
          
 
              Abstract 
 Aim:
              The present study was aimed to isolate and evaluate the 
              continuous change in the pattern of drug resistance showed by 
              different mastitogenic organisms, isolated from clinical and 
              subclinical cases of mastitis. 
              Materials and Methods: The study was carried out using 150 
              milk samples received from various clinical and subclinical cases, 
              from which the causative organisms were isolated and subjected to
              in vitro antibiotic sensitivity test. 
              Results: The bacteriological analysis of the samples indicated 
              the presence of both Gram positive and Gram negative organisms 
              followed by isolation of isolates like Staphylococcus, E. coli, 
              Streptococcus, Bacillus, Corynebacterium, Listeria, Klebsiella. 
              The in vitro sensitivity of Staphylococcus, E. 
              coli and Streptococcus isolates revealed that they were 
              more sensitive towards newer antimicrobials like Levofloxacin and 
              Enrofloxacin. 
              Conclusion: The prevalence of Staphylococcus was found 
              to be maximum followed by Streptococcus and E. coli 
              among the isolated organisms. Levofloxacin and Enrofloxacin were 
              found to be most effective against the targeted isolates. Key 
              words: antibiotic sensitivity pattern, bovine, mastitis, milk. 
 
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