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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online : 14-07-2013)
18.
Evaluation of live gumboro vaccine prepared from
local variant strain for control of infectious bursal disease in Egypt -
Susan S. El-Mahdy, Manal, A. Afify and A. M. Helal
Vet World. 2013; 6(9):
674-680
doi:
10.14202/vetworld.2013.674-680
Abstract
Aim:
The present study was designed to evaluate live Gumboro (IBD)
vaccine prepared from local variant isolated strain (Egy-IBD Var
2009 Vp2 gene-, partial cds submitted in gen bank at accession no.
: JN 118617) for controlling IBD problem in Egypt.
Material and Methods: Local isolated variant strain was
adapted on Baby Grivet Monkey kidney cell line -70(BGM-70) used
for preparation of tissue culture (T.C) live vaccine. T.C IBDV had
a titer of (10) 7.5
TCID50/ml ((10)4.5
TCID50 per dose) after five passages in BGM-70 cell
culture. Evaluation of prepared vaccine was done in vitro by
measuring ELISA, and in vivo by protection % against very virulent
or variant field IBD isolated strains.
Result:
Evaluation revealed that the prepared vaccine was safe;
sterile; pure; non-immunosuppressive; and efficient. The Geometric
Mean Titer (GMT) of ELISA for the prepared vaccine was 8271 and
more than 10000 in compared with different commercial IBD
vaccines; while protection percentage gave 96-100%; 92-96% and
96-98% in groups vaccinated with commercial (intermediate;
intermediate plus and classical) IBD vaccines; respectively in
compared to 96-100% in group vaccinated with local prepared
vaccine when challenged with very virulent or variant IBD isolated
strains.
Conclusion: We can use live T.C. IBD vaccine prepared from
local variant isolated virus strain as method for control IBD
disease in Egypt.
Keywords: Local variant infectious bursal disease virus (IBDV),
live vaccine, tissue culture
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