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Research (Published
online : 18-06-2013)
3. Safety
evaluation of seabuckthorn (Hippophae rhamnoides) leaves in Japanese quail
- Vikram Patial, R. K. Asrani and Rajendra Damu Patil
Vet World. 2013; 6(9):
596-600
doi:
10.5455/vetworld.2013.596-600
Abstract
Aim: The present study was
designed to evaluate the safety of seabuckthorn (SBT) (Hippophae
rhamnoides L. Elaeagnaceae) leaves added in the diet of Japanese
quail.
Materials and Methods: A total of ninety quail chicks were
randomly divided into 2 groups and fed a standard quail chick mash
added with or without 2% SBT leaf powder (Group SX), and a
standard quail chick mash (Group CX, control) for 21 days. Six
animals from each group were randomly sacrificed at 7, 14 and 21
day post feeding. Blood and tissue samples were collected for
biochemical and histopathological studies.
Results: The inclusion of SBT in quail diet did not affect
the weekly body weights, clinical signs or mortality among the
birds. The serum biochemical parameters like alanine transaminase,
total protein, albumin, cholesterol and uric acid values in SBT
treated group were found at par with those in the control group.
Gross and histopathological studies of various internal organs
such as liver, kidneys, heart, spleen, proventriculus, small
intestine, bursa and thymus did not reveal any significant lesions
in the tissue sections. However, proliferation of lymphoid tissue
was a prominent finding noted in different lymphoid organs.
Conclusion: In conclusion, the addition of seabuckthorn
leaf powder at 2% level in the diet of Japanese quail was found to
be safe and it can be used as a feed additive.
Keywords: biochemical changes, Japanese quail, pathology,
seabuckthorn
