Open Access 
              
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, 
distribution and reproduction in any medium, provided the work is properly 
cited.
              
              
              
              
              Research (Published 
online : 18-06-2013)
              
              
              4. Isolation and characterization of bacteria from canine 
              respiratory diseases in and around Hyderabad city, Andhra Pradesh, 
              India - S. Ayodhya, D. S. Tirumala Rao, Y. Narsimha 
              Reddy, N. Syam Sundar and V. Girish Kumar
Vet World. 2013; 6(9):
  601-604
              
               
              
              doi: 
              10.5455/vetworld.2013.601-604
               
              
  
  
   
  
              
               
               
               
              
              
              Abstract
              
              
              Aim: To identify the 
              organisms causing respiratory diseases in dogs that belongs to 
              areas in and around Hyderabad city.
              Materials and Methods: Nasal samples were collected using 
              sterile swabs from the canine clinical cases that were presented 
              with the history of respiratory diseases at the University 
              administered Veterinary Hospital, Bhoiguda, Secunderabad and 
              Campus Veterinary Hospital, College of Veterinary Science, 
              Rajendranagar, Hyderabad over a period of 26 months i.e., from 
              October 2008 to November 2010. Clinical signs included coughing, 
              nasal discharge, sneezing, difficulty in breathing, fever, loss of 
              appetite and lethargic behavior. Nasal swabs were immediately 
              transferred, aseptically, into a nutrient broth and were subjected 
              to various cultural tests and microscopic examination. 
              Results: Out of 90 nasal samples, different species of 
              bacteria were isolated. Results from our bacterial culture, 
              microscopic and biochemical studies of the nasal discharges 
              collected from dogs suffering with respiratory diseases revealed 
              various bacteria such as E. coli, Klebsiella spp, Streptococci spp. 
              Staphylococci and mixed infections.
              Conclusion: This study revealed that the bacteria such as 
              E. coli, Klebsiella spp, Streptococci spp. Staphylococci and mixed 
              infections are the common causative agents that lead to 
              respiratory diseases in dogs belonging to areas in and around 
              Hyderabad city. 
              Keywords: bacteriology, characterization, cultural tests, 
              dogs, isolation, respiratory diseases