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Research
(Published
online: 21-04-2014)
14. The evaluation of metabolizable
protein content of some indigenous feedstuffs used in ruminant
nutrition - Lalatendu Keshary Das, S. S. Kundu, Dinesh
Kumar and Chander Datt
Veterinary World, 7(4): 257-261
doi:
10.14202/vetworld.2014.257-261
Lalatendu Keshary Das: Veterinary Dispensary, Kalampur,
Kalahandi, Odisha, India.
S. S. Kundu: Division of Dairy Cattle Nutrition, National
Dairy Research Institute, Karnal, Haryana, India.
Dinesh Kumar: Division of Animal Nutrition, Indian
Veterinary Research Institute, Izatnagar, Uttar Pradesh, India
Chander Datt: Division of Dairy Cattle Nutrition, National
Dairy Research Institute, Karnal, Haryana, India.
Received: 01-02-2014, Revised: 20-03-2014, Accepted: 25-03-2014,
Published online: 21-04-2014
Corresponding author:
Lalatendu Keshary Das, email: drlalatendu27@gmail.com
Abstract
Aim: To determine the
metabolizable protein (MP) content of common indigenous feedstuffs
used in ruminant nutrition using in situ method.
Materials and Methods: Nine ruminant feeds such as maize
grain (MG), groundnut cake (GNC), mustard oilcake (MOC),
cottonseed cake (CSC), deoiled rice bran (DORB), wheat bran (WB),
berseem fodder (BF), maize fodder (MF) and sorghum fodder (SF)
were included in this study. Each test feed was dried, ground and
chemically analysed for proximate principles (DM, CP, EE, OM,
Total ash), fiber fractions (NDF, ADF, cellulose, hemicellulose,
lignin), NDICP and ADICP. Two adult fistulated bulls were used for
evaluating the protein degradation characteristics of each test
feed using the nylon bag method. Metabolizable energy (ME) content
of the test feeds were predicted from their chemical composition
data using summative approach of NRC (2001) model. The equations
of AFRC (1992) were used to predict the rumen degradable protein (RDP),
digestible microbial protein (DMP), digestible undegraded feed
protein (DUP) and MP content of test feeds.
Results: The MP content of MG, GNC, MOC, CSC, DORB, WB, BF,
MF and SF was found to be 95.26, 156.41, 135.21, 125.06, 101.68,
107.11, 136.81, 72.01 and 76.65 g/kg DM, respectively. The
corresponding ME (MJ/kg DM) content of the test feeds was 13.66,
13.12, 13.65, 10.68, 9.08, 11.56, 9.64, 8.33 and 8.03,
respectively. Among the test feeds, GNC contained the highest and
MF contained the lowest MP per kg DM.
Conclusion: It was concluded that the degradability of
crude protein (CP) of the test feeds can be used in MP
determination and diet formulation. Feed CP content is not
available as such at intestinal level in ruminants as a definite
part of it undergoes extensive microbial degradation in rumen. The
pattern and extent of such degradation do influence the amount of
protein presented to lower digestive tract (MP) for absorption and
utilization in ruminants. It was also found that the MP content of
a feed is not constant as that of its CP content as it entirely
depends on the degradation characteristics in a given animal.
Keywords: in situ method, metabolizable energy,
metabolizable protein, microbial protein, ruminant nutrition.
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