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Research
(Published
online: 06-04-2014)
4. Evaluation and comparison of the constitutive expression
levels of Toll-like receptors 2, 3 and 7 in the peripheral blood
mononuclear cells of Tharparkar and crossbred cattle
- Lakshya Veer Singh, Shikha Saxena, Smita Gupta, Shishir Kumar
Gupta, G. Ravi Kumar, G. S. Desai, Aditya Prasad Sahoo, D. R.
Harish and A. K. Tiwari
Veterinary World, 7(4):
209-212
doi:
10.14202/vetworld.2014.209-212
Lakshya Veer Singh: Molecular Biology Laboratory, Division
of Veterinary Biotechnology, Indian Veterinary Research Institute,
Izatnagar, Bareilly-243122, Uttar Pradesh, India
Shikha Saxena:
Molecular Biology Laboratory, Division of Veterinary
Biotechnology, Indian Veterinary Research Institute, Izatnagar,
Bareilly-243122, Uttar Pradesh, India
Smita Gupta:
Molecular Biology Laboratory, Division of Veterinary
Biotechnology, Indian Veterinary Research Institute, Izatnagar,
Bareilly-243122, Uttar Pradesh, India
Shishir Kumar Gupta:
Molecular Biology Laboratory, Division of Veterinary
Biotechnology, Indian Veterinary Research Institute, Izatnagar,
Bareilly-243122, Uttar Pradesh, India
G. Ravi Kumar:
Molecular Biology Laboratory, Division of Veterinary
Biotechnology, Indian Veterinary Research Institute, Izatnagar,
Bareilly-243122, Uttar Pradesh, India
G. S. Desai:
Molecular Biology Laboratory, Division of Veterinary
Biotechnology, Indian Veterinary Research Institute, Izatnagar,
Bareilly-243122, Uttar Pradesh, India
Aditya Prasad Sahoo:
Molecular Biology Laboratory, Division of Veterinary
Biotechnology, Indian Veterinary Research Institute, Izatnagar,
Bareilly-243122, Uttar Pradesh, India
D. R. Harish:
Molecular Biology Laboratory, Division of Veterinary
Biotechnology, Indian Veterinary Research Institute, Izatnagar,
Bareilly-243122, Uttar Pradesh, India
A. K. Tiwari:
Molecular Biology Laboratory, Division of Veterinary
Biotechnology, Indian Veterinary Research Institute, Izatnagar,
Bareilly-243122, Uttar Pradesh, India
Received: 10-02-2014, Revised: 03-03-2014, Accepted: 08-03-2014,
Published online: 06-04-2014
Corresponding author: A. K. Tiwari, email: aktiwari63@yahoo.com
Abstract
Aim: This study was
undertaken to assess the differential expression levels of
toll-like receptors (TLRs) 2, 3 and 7 in peripheral blood
mononuclear cells (PBMCs) isolated from Tharparkar and Crossbred
cattle belonging to different regions of India.
Materials and Methods: PBMCs were isolated from blood
samples of Tharparkar cattle from Indian Veterinary Research
Institute (IVRI) farm (n=30); Suratgarh farm (n=61); Jaipur farm
(n=8) and cross breed cattle from Jaipur (n=47). RNA was isolated
from PBMCs and cDNA was synthesized using random hexamers. The
expression profiles of TLR 2, 3 and 7 were estimated by real-time
PCR and normalized to the expression of β-actin.
Results: PBMCs of Tharparkar cattle from Suratgarh,
exhibited a significantly higher (p<0.05) constitutive expression
levels of TLR2, TLR3 and TLR7 genes as compared to Tharparkar
cattle from IVRI or Jaipur as well as the crossbred cattle from
Jaipur. PBMCs of crossbred cattle from Jaipur showed higher
expression profiles of all the TLRs than Tharparkar cattle from
Jaipur and IVRI.
Conclusion: Our study indicates, expression levels of TLR2,
TLR3 and TLR7 are significantly higher for Tharparkar cattle from
Suratgarh than the cattle from Jaipur and IVRI and crossbred
cattle from Jaipur. However, crossbred cattle from Jaipur showed
higher basal expression levels of all the three TLRs than
Tharparkar cattle from Jaipur and IVRI. Results also indicate that
PBMCs of Tharparkar cattle show a regional variation in the
expression pattern of TLRs.
Keywords: crossbred, immunity, resistance, Tharparkar
cattle, Toll-like receptors.
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