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Research
(Published
online: 10-04-2014)
7. Estimation of titers of antibody
against Pasteurella multocida in cattle vaccinated with
haemorrhagic septicemia alum precipitated vaccine -
Sabia Qureshi and Hari Mohan Saxena
Veterinary World, 7(4): 224-228
doi:
10.14202/vetworld.2014.224-228
Sabia Qureshi: Department of Veterinary
Microbiology, College of Veterinary Science, Guru Angad Dev
Veterinary and Animal Sciences University (GADVASU), Ludhiana,
India.
Hari Mohan Saxena: Department of Veterinary
Microbiology, College of Veterinary Science, Guru Angad Dev
Veterinary and Animal Sciences University (GADVASU), Ludhiana,
India.
Received: 17-01-2014, Revised: 09-03-2014, Accepted: 11-03-2014,
Published online: 10-04-2014
Corresponding author: Hari Mohan Saxena, email: hmsaxena@yahoo.com
Abstract
Aim: The present study was
carried out in 100 cattle to assess the antibody response to
Haemorrhagic Septicaemia alum precipitated vaccine by Microtiter
Agglutination Test (MAT), Indirect Haemaaglutination Assay (IHA)
and Monoclonal Antibody based Indirect Enzyme Linked Immunosorbent
Assay (ELISA).
Materials and Methods: One hundred cattle from a local
gaushala of Ludhiana were vaccinated with alum precipitated
Haemorrhagic Septicaemia vaccine. Serum was collected at 0, 42, 84
and 128 days post immunization (DPI) and antibody titers at different
stages were estimated by MAT, IHA and ELISA.
Results: The animals exhibited the classical pattern of
humoral immune response with gradual increase and achievement of
peak antibody titers plateau by 42 DPI and gradual decline by 128
DPI. The IHA titers in cattle were significantly higher (P<0.05)
at 42 days post immunization than the corresponding MAT titers on
the same day. ELISA titers were significantly higher (P<0.05) than
MAT and IHA titers at 42 DPI. IHA was found to be more sensitive
than MAT, and the titers were higher by ELISA than by MAT and IHA
throughout the observation period.
Conclusion: The results indicate that animals vaccinated
with commercial alum precipitated HS vaccine could not develop and
sustain adequate levels of antibody for long duration.
Keywords: antibody titers, haemorrhagic septicaemia,
Indirect Haemaaglutination Assay, Pasteurella multocida.
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