Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online: 21-08-2014)
12.
Serotyping
of Escherichia coli isolated from piglet diarrhea -
M.
Regon, D. C. Pathak, S. M. Tamuli and G. K. Baruah
Veterinary World, 7(8): 614-616
doi:
10.14202/vetworld.2014.614-616
M. Regon:
Department of Pathology, College of Veterinary Science, Assam
Agricultural University, Khanapara, Guwahati - 781 022,
Assam,
India;
manjularegon@gmail.com
D. C. Pathak:
Department of Pathology, College of Veterinary Science, Assam
Agricultural University, Khanapara, Guwahati - 781 022,
Assam,
India;
dcpathak@gmail.com
S. M. Tamuli:
Department of Pathology, College of Veterinary Science, Assam
Agricultural University, Khanapara, Guwahati - 781 022,
Assam,
India;
tamuli_sm@yahoo.com
G. K. Baruah:
Department of Pathology, College of Veterinary Science, Assam
Agricultural University, Khanapara, Guwahati - 781 022,
Assam,
India;
gkbaruah53@gmail.com
Received: 11-05-2014, Revised: 11-07-2014, Accepted: 16-07-2014,
Published online: 21-08-2014
Corresponding author: Monjula Regon, email:
manjularegon@gmail.com
Abstract
Aim:
The aim was to investigate the different strains of
Escherichia coli isolated from diarrheic piglets by
serological typing.
Materials and Methods: A total of 150 isolates consists of 66
from diarrheic feces and 84 from intestinal contents were
subjected to serological typing. The isolates were referred to
National Salmonella and E. coli Center, CRI, Kasauli,
Himachal Pradesh, India for serotyping.
Result:
Of 150 isolates, 90 isolates were serotyped into 20 different
serogroups, 4 isolates were rough and remaining 56 isolates were
refractory to serotype. The most frequently encountered serogroups
were O76 (25 strains), O60 (18 strains), O120 (11 strains), O87 (6
strains), O107 and O80 (5 strains each), O84 and O64 (3 strains
each), and O117 and O158 (2 strains each). The other serogroups
identified were O3, 05, O24, O25, O36, O42, O100, O116, 0132, and
O140 (1 strain each), 3 rough and 56 strains were untypable.
Conclusion: The results in the present study showed that
variable strains of E. coli are responsible for diarrhea in
piglets.
Keywords: diarrhea, Escherichia coli,
piglet, serotype.
References
1. Moxley, R.A. and Duhamel, G.E. (1999) Comparative pathology
of bacterial enteric diseases of swine. Adv. Exp. Med. Biol.,
473: 83-101.
http://dx.doi.org/10.1007/978-1-4615-4143-1_7
PMid:10659346 |
|
|
2. Zhang, W., Berberrow, E.M., Freeling, J., He, D., Morley,
R.A. and Francis, D.H. (2006) Significance of heat labile and
heat stable enterotoxins in porcine colibacillosis in an
additive model for pathogenecity study. Infect. Immun., 74(6):
3107-3114.
http://dx.doi.org/10.1128/IAI.01338-05
PMid:16714538 PMCid:PMC1479275 |
|
|
3. Fleckenstein, J.M., Hardwidge, P.R., Munson, G.P., Rasko,
D.A., Sommefelt, H. and Steinsland, H. (2010) Molecular
mechanism of enterotoxigenic Escherichia coli infection.
Microbes. Infect., 12: 89-98.
http://dx.doi.org/10.1016/j.micinf.2009.10.002
PMid:19883790 |
|
|
4. Madoroba, E., van Driessche, E., De Greve, H., Mast, J.,
Ncube, I., Read, J. and Beeckmans, S. (2009) Prevalence of
enterotoxigenic Escherichia coli virulence gene from scouring
piglets in Zimbabew. Trop. Anim. Health Prod., 41: 1539-1547.
http://dx.doi.org/10.1007/s11250-009-9345-4
PMid:19347597 |
|
|
5. Vu-Khac, H., Holoda, E., Pilipcinec, E., Blanco, M.,
Blanco, J.E., Dahbi, G., Mora, A., Lopez, C., Gonzalez, E.A.
and Blanco, J. (2007) Serotypes, virulence genes, intimin
types and PFGE profiles of Escherichia coli isolated from
piglets with diarrhoea in Slovakia. Vet. J., 174(1): 176-187.
http://dx.doi.org/10.1016/j.tvjl.2006.05.019
PMid:16956777 |
|
|
6. Kim, Y.J., Kim, J.H., Hur, J. and Le, J.H. (2010) Isolation
of Escherichia coli from piglets in South Korea with diarrhoea
and characteristics of the virulence genes. Can. J. Vet. Res.,
74(1): 59-64.
PMid:20357961 PMCid:PMC2801314 |
|
|
|
7. Collins, C.H. and Lyne, P.M. (1970) Microbiological
Methods. Laboratory technique series. University Park Press,
Baltimore, London. |
|
|
|
8. Cruickshank, R., Duguid, J.P., Marimion, B.P. and Swain,
R.H.A. (1975). Medical Microbiology. 12th ed., Vol. II.
Churchill Livingstone, Edinburgh, London and New York. |
|
|
9. Frydendahl, K. (2002) Prevalence of serogroups and
virulence genes in Escherichia coli associated with
postweaning diarrhoea and edema disease in pigs and a
comparison of diagnostic diagnostic approaches. Vet. Microbiol.,
85: 169-182.
http://dx.doi.org/10.1016/S0378-1135(01)00504-1 |
|
|
10. Chen, X., Gao, S., Jiao, X. and Liu, X.F. (2004)
Prevalence of serogroups and virulence factors of Escherichia
coli strains isolated from pigs with postweaning diarrhoea in
eastern China. Vet. Microbiol., 103(1-2): 13-20.
http://dx.doi.org/10.1016/j.vetmic.2004.06.014
PMid:15381261 |
|
|
|
11. Roychoudhury, P., Saikia, G.K and Rahman, H. (2002)
Serotyping antibiogram of Escherichia coli isolated from
diarrhoeic piglets. Indian J. Anim. Sci., 74(1): 41-42. |
|
|
12. Fratamico Pina, M., Bagi Lori, K., Bush Eric, J. and
Barbara, T. (2004) Prevalence and characterization of shiga
toxin-producing Escherichia coli in swine faeces recovered in
the National Animal Health Monitoring System's Swine 2000
Study. Appl. Env. Microbiol., 70(12): 7173-7178.
http://dx.doi.org/10.1128/AEM.70.12.7173-7178.2004
PMid:15574914 PMCid:PMC535163 |
|
|
|
13. Byun, J.W., Jung, B.Y., Kim, H.Y., Fairbrother, J.M. Lee,
R.M.H. and Lee, W.K. (2013) O-serogroups, virulence genes of
pathogenic Escherichia coli and Pulsed-field gel
electrophoresis (PFGE) patterns of O149 isolates from
diarrhoeic piglets in Korea. Vet. Med., 58(9): 468-476. |
|
|
|
14. Xuefeng, Q., Huang, N., Zhao, B., Chungjiang, W. and Zhao,
X. (2012) Prevalence of serogropus and genotypes for fimbriae
and enterotoxin in Escherichia coli isolated from diarrhoeic
piglet in Western China. J. Swine Health Prod., 20(6):
290-293. |
|
|
15. Harel, J., Lapointe, H., Fallara, A., Lortie, L.A.,
Bigras-Poulin, M., Lariviere, S. and Fairbrother, J.M. (1991)
Detection of genes for fimbrial antigens and enterotoxins
associated with Escherichia coli serogroups isolated from pigs
with diarrhoea. J. Clin. Microbiol., 29(4): 745-752.
PMid:1679765 PMCid:PMC269864 |
|
|
16. Fairbrother, J.M., Nadeau, E. and Gyles, C.L. (2005)
Escherichia coli in postweaning diarrhoea in pigs: An update
on bacterial types, pathogenesis, and prevention strategies.
Anim. Health Res. Rev., 6: 17-39.
http://dx.doi.org/10.1079/AHR2005105
PMid:16164007 |
|
|
|
17. Andrea, T., Daniela, G., Celene, C., Rosalba, C., Jorge,
L., Silvia, G. and Ana Marıa, C. (2012) Prevalence of
virulence genes in Escherichia coli strains isolated from
piglets in the suckling and weaning period in Mexico. J Med.
Microbiol. 61(Pt 1): 148-156. |
|
